Investigating lasp-2 in cell adhesion: New binding partners and roles in motility

Katherine T. Bliss, Miensheng Chu, Colin M. Jones-Weinert, Carol Gregorio

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Focal adhesions are intricate protein complexes that facilitate cell attachment, migration, and cellular communication. Lasp-2 (LIM-nebulette), a member of the nebulin family of actin-binding proteins, is a newly identified component of these complexes. To gain further insights into the functional role of lasp-2, we identified two additional binding partners of lasp-2: the integral focal adhesion proteins vinculin and paxillin. Of interest, the interaction of lasp-2 with its binding partners vinculin and paxillin is significantly reduced in the presence of lasp-1, another nebulin family member. The presence of lasp-2 appears to enhance the interaction of vinculin and paxillin with each other; however, as with the interaction of lasp-2 with vinculin or paxillin, this effect is greatly diminished in the presence of excess lasp-1. This suggests that the interplay between lasp-2 and lasp-1 could be an adhesion regulatory mechanism. Lasp-2's potential role in metastasis is revealed, as overexpression of lasp-2 in either SW620 or PC-3B1 cells-metastatic cancer cell lines-increases cell migration but impedes cell invasion, suggesting that the enhanced interaction of vinculin and paxillin may functionally destabilize focal adhesion composition. Taken together, these data suggest that lasp-2 has an important role in coordinating and regulating the composition and dynamics of focal adhesions.

Original languageEnglish (US)
Pages (from-to)995-1006
Number of pages12
JournalMolecular Biology of the Cell
Volume24
Issue number7
DOIs
StatePublished - Apr 1 2013

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Paxillin
Vinculin
Cell Adhesion
Focal Adhesions
Cell Movement
Microfilament Proteins
Proteins
Communication
Neoplasm Metastasis
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Investigating lasp-2 in cell adhesion : New binding partners and roles in motility. / Bliss, Katherine T.; Chu, Miensheng; Jones-Weinert, Colin M.; Gregorio, Carol.

In: Molecular Biology of the Cell, Vol. 24, No. 7, 01.04.2013, p. 995-1006.

Research output: Contribution to journalArticle

Bliss, Katherine T. ; Chu, Miensheng ; Jones-Weinert, Colin M. ; Gregorio, Carol. / Investigating lasp-2 in cell adhesion : New binding partners and roles in motility. In: Molecular Biology of the Cell. 2013 ; Vol. 24, No. 7. pp. 995-1006.
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