Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae

Trevor R. Jarman, A. A.Leslie Gunatilaka, David A. Widdowson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.

Original languageEnglish (US)
Pages (from-to)202-211
Number of pages10
JournalBioorganic Chemistry
Volume4
Issue number2
DOIs
StatePublished - Jun 1975
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

Cite this