Involvement of site-specific FAK phosphorylation in sphingosine-1 phosphate- and thrombin-induced focal adhesion remodeling: Role of Src and GIT

Yasushi Shikata, Konstantin G. Birukov, Anna A. Birukova, Alexander Verin, Joe GN Garcia

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Sphingosine-1 phosphate (S1P) and thrombin are agents with profound but divergent effects on vascular endothelial cell (EC) barrier properties. We have previously reported that S1P-induced focal adhesion (FA) remodeling involves interactions between focal adhesion kinase (FAK), paxillin, and G-protein-coupled receptor kinase-interacting proteins GIT1 and GIT2 and suggested a critical involvement of focal adhesions in the EC barrier regulation. In this study, we examined redistribution of FA proteins (FAK, paxillin, GIT1, and GIT2) and site-specific FAK tyrosine phosphorylation in human pulmonary artery endothelial cells stimulated with thrombin. In contrast to S1P, which we have shown to induce peripheral translocation of FA proteins associated with cortical actin ring formation, thrombin caused the redistribution of FA proteins to the ends of the newly formed massive stress fibers. S1P and thrombin induced distinct patterns of FAK site-specific phosphorylation with the FAK y576 phosphorylation site targeted by SIP challenge and phosphorylation of three FAK sites (Y397, Y 576, and Y925) in response to thrombin stimulation. Pharmacological inhibition of Src with Src-specific inhibitor PP2 abolished S1P-induced translocation of FA proteins, cortical actin ring formation, and FAK [Y576] phosphorylation. However, PP2 failed to alter thrombin-induced morphological changes and exhibited only partial inhibition of FAK site-specific tyrosine phosphorylation. These observations highlight the differential mechanisms of focal adhesion protein complex remodeling and FAK activation by S1P and thrombin and link differential FA remodeling to EC barrier regulation.

Original languageEnglish (US)
Pages (from-to)2240-2249
Number of pages10
JournalFASEB Journal
Volume17
Issue number15
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

non-specific protein-tyrosine kinase
sphingosine
Focal Adhesion Protein-Tyrosine Kinases
Phosphorylation
Focal Adhesions
thrombin
Thrombin
adhesion
phosphorylation
Adhesion
phosphates
Endothelial cells
endothelial cells
Endothelial Cells
Paxillin
Proteins
proteins
actin
Tyrosine
tyrosine

Keywords

  • Barrier function
  • Cytoskeleton
  • Human pulmonary artery endothelium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Involvement of site-specific FAK phosphorylation in sphingosine-1 phosphate- and thrombin-induced focal adhesion remodeling : Role of Src and GIT. / Shikata, Yasushi; Birukov, Konstantin G.; Birukova, Anna A.; Verin, Alexander; Garcia, Joe GN.

In: FASEB Journal, Vol. 17, No. 15, 12.2003, p. 2240-2249.

Research output: Contribution to journalArticle

Shikata, Yasushi ; Birukov, Konstantin G. ; Birukova, Anna A. ; Verin, Alexander ; Garcia, Joe GN. / Involvement of site-specific FAK phosphorylation in sphingosine-1 phosphate- and thrombin-induced focal adhesion remodeling : Role of Src and GIT. In: FASEB Journal. 2003 ; Vol. 17, No. 15. pp. 2240-2249.
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