Iodination associated inactivation of β-melanocyte stimulating hormone

Christopher B. Heward, Young C S Yang, Tomi K. Sawyer, Marvin D. Bregman, Bryan B. Fuller, Victor J Hruby, Mac E. Hadley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Contrary to other published reports, iodinated β-melanocyte stimulating hormone (β-MSH) is without biological activity as measured by frog skin bioassay, melanoma (mouse S-91) adenylate cyclase assay, or melanoma tyrosinase assay. Inactivation results in part from oxidation of the methionine residue by the chloramine T and sodium metabisulfite used in the iodination reaction. Replacement of the methionine of β-MSH with norleucine by solid phase synthesis results in an analogue which is more resistant, but not completely resistant, to inactivation. Thus, in order to obtain a biologically active radioligand for radioreceptor studies, further tailoring of the hormone and/or modification of the iodination procedure will be needed.

Original languageEnglish (US)
Pages (from-to)266-273
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume88
Issue number1
DOIs
StatePublished - May 14 1979

Fingerprint

Melanocyte-Stimulating Hormones
Halogenation
Methionine
Melanoma
Assays
Norleucine
Solid-Phase Synthesis Techniques
Monophenol Monooxygenase
Bioassay
Bioactivity
Adenylyl Cyclases
Biological Assay
Anura
Skin
Hormones
Oxidation
chloramine-T
sodium metabisulfite

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Heward, C. B., Yang, Y. C. S., Sawyer, T. K., Bregman, M. D., Fuller, B. B., Hruby, V. J., & Hadley, M. E. (1979). Iodination associated inactivation of β-melanocyte stimulating hormone. Biochemical and Biophysical Research Communications, 88(1), 266-273. https://doi.org/10.1016/0006-291X(79)91725-X

Iodination associated inactivation of β-melanocyte stimulating hormone. / Heward, Christopher B.; Yang, Young C S; Sawyer, Tomi K.; Bregman, Marvin D.; Fuller, Bryan B.; Hruby, Victor J; Hadley, Mac E.

In: Biochemical and Biophysical Research Communications, Vol. 88, No. 1, 14.05.1979, p. 266-273.

Research output: Contribution to journalArticle

Heward, Christopher B. ; Yang, Young C S ; Sawyer, Tomi K. ; Bregman, Marvin D. ; Fuller, Bryan B. ; Hruby, Victor J ; Hadley, Mac E. / Iodination associated inactivation of β-melanocyte stimulating hormone. In: Biochemical and Biophysical Research Communications. 1979 ; Vol. 88, No. 1. pp. 266-273.
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