Ion channel probes for scanning ion conductance microscopy

Yi Zhou, Leonard K. Bright, Wenqing Shi, Craig A. Aspinwall, Lane A. Baker

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The sensitivity and selectivity of ion channels provide an appealing opportunity for sensor development. Here, we describe ion channel probes (ICPs), which consist of multiple ion channels reconstituted into lipid bilayers suspended across the opening of perflourinated glass micropipets. When incorporated with a scanning ion conductance microscope (SICM), ICPs displayed a distance-dependent current response that depended on the number of ion channels in the membrane. With distance-dependent current as feedback, probes were translated laterally, to demonstrate the possibility of imaging with ICPs. The ICP platform yields several potential advantages for SICM that will enable exciting opportunities for incorporation of chemical information into imaging and for high-resolution imaging.

Original languageEnglish (US)
Pages (from-to)15351-15355
Number of pages5
JournalLangmuir
Volume30
Issue number50
DOIs
StatePublished - Dec 23 2014

    Fingerprint

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Zhou, Y., Bright, L. K., Shi, W., Aspinwall, C. A., & Baker, L. A. (2014). Ion channel probes for scanning ion conductance microscopy. Langmuir, 30(50), 15351-15355. https://doi.org/10.1021/la504097f