Islet assessment for transplantation

Klearchos K. Papas, Thomas M. Suszynski, Clark K. Colton

Research output: Contribution to journalReview article

62 Scopus citations

Abstract

Purpose of review: There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation. Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most Recent findings:results while focusing on the most promising assays. Recent findings: Assays based on membrane integrity do not reflect true viability when applied to either intact islets or dispersed islet cells. Assays requiring disaggregation of intact islets into individual cells for assessment introduce additional problems of cell damage and loss. Assays evaluating mitochondrial function, specifically mitochondrial membrane potential, bioenergetic status, and cellular oxygen consumption rate, especially when conducted with intact islets, appear most promising in evaluating their quality prior to islet cell transplantation. Prospective, quantitative assays based on measurements of oxygen consumption rate with intact islets have been developed, validated, and their results correlated with transplant outcomes in the diabetic nude mouse bioassay. Conclusion: More sensitive and reliable islet viability and potency tests have been recently developed and tested. Those evaluating mitochondrial function are most promising, correlate with transplant outcomes in mice, and are currently being evaluated in the clinical setting.

Original languageEnglish (US)
Pages (from-to)674-682
Number of pages9
JournalCurrent Opinion in Organ Transplantation
Volume14
Issue number6
DOIs
StatePublished - Dec 2009

Keywords

  • Marginal mass
  • Oxygen consumption rate
  • Potency
  • Purity
  • Release criteria
  • Viability

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation

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