Islet assessment for transplantation

Klearchos K Papas, Thomas M. Suszynski, Clark K. Colton

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Purpose of review: There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation. Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most Recent findings:results while focusing on the most promising assays. Recent findings: Assays based on membrane integrity do not reflect true viability when applied to either intact islets or dispersed islet cells. Assays requiring disaggregation of intact islets into individual cells for assessment introduce additional problems of cell damage and loss. Assays evaluating mitochondrial function, specifically mitochondrial membrane potential, bioenergetic status, and cellular oxygen consumption rate, especially when conducted with intact islets, appear most promising in evaluating their quality prior to islet cell transplantation. Prospective, quantitative assays based on measurements of oxygen consumption rate with intact islets have been developed, validated, and their results correlated with transplant outcomes in the diabetic nude mouse bioassay. Conclusion: More sensitive and reliable islet viability and potency tests have been recently developed and tested. Those evaluating mitochondrial function are most promising, correlate with transplant outcomes in mice, and are currently being evaluated in the clinical setting.

Original languageEnglish (US)
Pages (from-to)674-682
Number of pages9
JournalCurrent Opinion in Organ Transplantation
Volume14
Issue number6
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

Islets of Langerhans Transplantation
Islets of Langerhans
Cell Transplantation
Oxygen Consumption
Transplants
Mitochondrial Membrane Potential
Nude Mice
Biological Assay
Energy Metabolism
Membranes

Keywords

  • Marginal mass
  • Oxygen consumption rate
  • Potency
  • Purity
  • Release criteria
  • Viability

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation

Cite this

Islet assessment for transplantation. / Papas, Klearchos K; Suszynski, Thomas M.; Colton, Clark K.

In: Current Opinion in Organ Transplantation, Vol. 14, No. 6, 12.2009, p. 674-682.

Research output: Contribution to journalArticle

Papas, Klearchos K ; Suszynski, Thomas M. ; Colton, Clark K. / Islet assessment for transplantation. In: Current Opinion in Organ Transplantation. 2009 ; Vol. 14, No. 6. pp. 674-682.
@article{7295ae1348824f71ba9fa7ad4e03d309,
title = "Islet assessment for transplantation",
abstract = "Purpose of review: There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation. Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most Recent findings:results while focusing on the most promising assays. Recent findings: Assays based on membrane integrity do not reflect true viability when applied to either intact islets or dispersed islet cells. Assays requiring disaggregation of intact islets into individual cells for assessment introduce additional problems of cell damage and loss. Assays evaluating mitochondrial function, specifically mitochondrial membrane potential, bioenergetic status, and cellular oxygen consumption rate, especially when conducted with intact islets, appear most promising in evaluating their quality prior to islet cell transplantation. Prospective, quantitative assays based on measurements of oxygen consumption rate with intact islets have been developed, validated, and their results correlated with transplant outcomes in the diabetic nude mouse bioassay. Conclusion: More sensitive and reliable islet viability and potency tests have been recently developed and tested. Those evaluating mitochondrial function are most promising, correlate with transplant outcomes in mice, and are currently being evaluated in the clinical setting.",
keywords = "Marginal mass, Oxygen consumption rate, Potency, Purity, Release criteria, Viability",
author = "Papas, {Klearchos K} and Suszynski, {Thomas M.} and Colton, {Clark K.}",
year = "2009",
month = "12",
doi = "10.1097/MOT.0b013e328332a489",
language = "English (US)",
volume = "14",
pages = "674--682",
journal = "Current Opinion in Organ Transplantation",
issn = "1087-2418",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Islet assessment for transplantation

AU - Papas, Klearchos K

AU - Suszynski, Thomas M.

AU - Colton, Clark K.

PY - 2009/12

Y1 - 2009/12

N2 - Purpose of review: There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation. Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most Recent findings:results while focusing on the most promising assays. Recent findings: Assays based on membrane integrity do not reflect true viability when applied to either intact islets or dispersed islet cells. Assays requiring disaggregation of intact islets into individual cells for assessment introduce additional problems of cell damage and loss. Assays evaluating mitochondrial function, specifically mitochondrial membrane potential, bioenergetic status, and cellular oxygen consumption rate, especially when conducted with intact islets, appear most promising in evaluating their quality prior to islet cell transplantation. Prospective, quantitative assays based on measurements of oxygen consumption rate with intact islets have been developed, validated, and their results correlated with transplant outcomes in the diabetic nude mouse bioassay. Conclusion: More sensitive and reliable islet viability and potency tests have been recently developed and tested. Those evaluating mitochondrial function are most promising, correlate with transplant outcomes in mice, and are currently being evaluated in the clinical setting.

AB - Purpose of review: There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation. Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most Recent findings:results while focusing on the most promising assays. Recent findings: Assays based on membrane integrity do not reflect true viability when applied to either intact islets or dispersed islet cells. Assays requiring disaggregation of intact islets into individual cells for assessment introduce additional problems of cell damage and loss. Assays evaluating mitochondrial function, specifically mitochondrial membrane potential, bioenergetic status, and cellular oxygen consumption rate, especially when conducted with intact islets, appear most promising in evaluating their quality prior to islet cell transplantation. Prospective, quantitative assays based on measurements of oxygen consumption rate with intact islets have been developed, validated, and their results correlated with transplant outcomes in the diabetic nude mouse bioassay. Conclusion: More sensitive and reliable islet viability and potency tests have been recently developed and tested. Those evaluating mitochondrial function are most promising, correlate with transplant outcomes in mice, and are currently being evaluated in the clinical setting.

KW - Marginal mass

KW - Oxygen consumption rate

KW - Potency

KW - Purity

KW - Release criteria

KW - Viability

UR - http://www.scopus.com/inward/record.url?scp=74349099562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74349099562&partnerID=8YFLogxK

U2 - 10.1097/MOT.0b013e328332a489

DO - 10.1097/MOT.0b013e328332a489

M3 - Article

C2 - 19812494

AN - SCOPUS:74349099562

VL - 14

SP - 674

EP - 682

JO - Current Opinion in Organ Transplantation

JF - Current Opinion in Organ Transplantation

SN - 1087-2418

IS - 6

ER -