Isolation and characterization of a partial cDNA for a human sialyltransferase

Michael P Lance, Karen M. Lau, Joseph T Y Lau

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A probe generated from the coding sequence of the rat hepatic β-galactoside α2,6-sialyltransferase was used to screen a human cDNA library constructed of human submaxillary gland mRNA lambda gt-11. We report the isolation and characterization of a human cDNA, HSM-ST1, that is putatively the human homolog of the β-galactoside α2,6-sialyltransferase. The largest human clone contains a 1.3 kb cDNA insert and is predicted to encompass 75% of the coding sequence as well as a small portion of the 3′ untranslated region. Comparative analysis of this insert with the rat hepatic α2,6-sialyltransferase sequence indicates 79% nucleotide similarity between the two sequences in the predicted coding region. On the amino acid level, the degree of conservation is 86%. Substantial sequence similarity is observed in the 3′-untranslated region between the rat and human sequences as well. S1 nuclease analysis was performed to demonstrate the expression of HSM-ST1 transcripts in the human hepatoma cell line, HepG2, and in the human colonic adenocarcinoma cell lines, LS174T.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number1
DOIs
StatePublished - Oct 16 1989
Externally publishedYes

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Sialyltransferases
Rats
Galactosides
Complementary DNA
3' Untranslated Regions
Cells
Gene Library
Conservation
Nucleotides
Amino Acids
Messenger RNA
Cell Line
Submandibular Gland
Liver
Hepatocellular Carcinoma
Adenocarcinoma
Clone Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation and characterization of a partial cDNA for a human sialyltransferase. / Lance, Michael P; Lau, Karen M.; Lau, Joseph T Y.

In: Biochemical and Biophysical Research Communications, Vol. 164, No. 1, 16.10.1989, p. 225-232.

Research output: Contribution to journalArticle

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