Isolation and characterization of Abelson murine leukemia virus‐transformed mast cell lines from midgestation embryonic placenta

Michael L. Siegel, Randy J Horwitz, Timothy D. Morris, Raina M. Di Venere, Edward J. Siden

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Abelson murine leukemia virus was used to transform cells of the midgestation embryonic placenta. The frequency of transformed foci in semisolid agarose was highest when cells were isolated at 10 days of gestation and cell lines could be established in liquid culture. The continuous cell lines express characteristics of cultured mast cells, including surface antigens which are shared with lymphocytes and mono‐ nuclear phagocytes. These results imply a relationship between the transforming gene product and the mast cell growth factor interleukin 3.

Original languageEnglish (US)
Pages (from-to)1136-1141
Number of pages6
JournalEuropean Journal of Immunology
Volume15
Issue number11
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Placenta
Leukemia
Abelson murine leukemia virus
Cell Line
Stem Cell Factor
Interleukin-3
Surface Antigens
Phagocytes
Oncogenes
Mast Cells
Sepharose
Cultured Cells
Lymphocytes
Pregnancy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Isolation and characterization of Abelson murine leukemia virus‐transformed mast cell lines from midgestation embryonic placenta. / Siegel, Michael L.; Horwitz, Randy J; Morris, Timothy D.; Di Venere, Raina M.; Siden, Edward J.

In: European Journal of Immunology, Vol. 15, No. 11, 01.01.1985, p. 1136-1141.

Research output: Contribution to journalArticle

Siegel, Michael L. ; Horwitz, Randy J ; Morris, Timothy D. ; Di Venere, Raina M. ; Siden, Edward J. / Isolation and characterization of Abelson murine leukemia virus‐transformed mast cell lines from midgestation embryonic placenta. In: European Journal of Immunology. 1985 ; Vol. 15, No. 11. pp. 1136-1141.
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