Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana

J. P. McNevin, W. Woodward, A. Hannoufa, Kenneth A Feldmann, B. Lemieux

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Thirteen Arabidopsis thaliana mutants with deviating epicuticular wax layers (i.e., cer mutants) were isolated by screening 13000 transformed lines produced by the seed transformation method. After crossing the 13 mutants to some of the previously known cer mutant lines, 12 of our mutants mapped to 6 of the 21 known complementation groups (cer1 through cer4 as well as cer6 and cer10), while the other mutant corresponded to a previously unknown locus, cer21. Mutant phenotypes of 6 of the 13 mutant lines were caused by T-DNA insertions within cer genes. We also analyzed the chemical composition of the epicuticular wax layers of the cer mutants isolated in this study relative to that of Arabidopsis wild-type plants. Our results suggest that the five genes we tagged regulate different steps in wax biosynthesis, i.e., the decarbonylation of fatty aldehydes to alkanes, the elongation of hexacosanoic acid to octacosanoic acid, the reduction of fatty aldehydes to primary alcohols and the production of free aldehydes, while an insertion in the fifth gene causes an alteration in the chain length distribution of the different classes of wax compounds.

Original languageEnglish (US)
Pages (from-to)610-618
Number of pages9
JournalGenome
Volume36
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Waxes
Arabidopsis
Arabidopsis thaliana
mutants
DNA
Genes
aldehydes
Alkanes
epicuticular wax
Aldehydes
waxes
Seeds
Alcohols
Phenotype
T-DNA
genes
acids
alkanes
alcohols
chemical composition

Keywords

  • epicuticular wax
  • gas chromatography-mass spectroscopy
  • glossy mutants

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana. / McNevin, J. P.; Woodward, W.; Hannoufa, A.; Feldmann, Kenneth A; Lemieux, B.

In: Genome, Vol. 36, No. 3, 1993, p. 610-618.

Research output: Contribution to journalArticle

McNevin, JP, Woodward, W, Hannoufa, A, Feldmann, KA & Lemieux, B 1993, 'Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana', Genome, vol. 36, no. 3, pp. 610-618.
McNevin, J. P. ; Woodward, W. ; Hannoufa, A. ; Feldmann, Kenneth A ; Lemieux, B. / Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana. In: Genome. 1993 ; Vol. 36, No. 3. pp. 610-618.
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