Isolation and characterization of human placental chorionic villar extracellular matrix

M. Ohno, Raymond B Nagle, E. Meezan, K. Brendel

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

The cell-free extracellular matrix of human placenta chorionic villi has been prepared by a procedure employing extraction of the terminal villar fragments with the detergents. Triton X-100 and sodium deoxycholate. The isolated human placental extracellular matrix retains an intact, but collapsed, histoarchitecture, as observed by scanning and transmission electron microscopy. It remains intact, in large part because of the presence of continous sheets of villar basement membranes and associated interstitial collagen fibers and scattered patches of fibrin. The staining characteristics and chemical composition of the isolated human placental extracellular matrix are similar to those reported for basement membranes in several tissues and indicate the presence of collagen-like and glycoprotein components in this preparation. Gel electrophoresis of urea-SDS-mercaptoethanol extracts of the matrix showed that it consists of several polypeptide components of various molecular weights, some of which are associated into high molecular weight complexes by disulfide bonds.

Original languageEnglish (US)
Title of host publicationJournal of Supramolecular and Cellular Biochemistry
Pages457-466
Number of pages10
Volume12
Edition4
StatePublished - 1979

Fingerprint

Extracellular Matrix
Collagen
Molecular weight
Basement Membrane
Deoxycholic Acid
Mercaptoethanol
Octoxynol
Molecular Weight
Fibrin
Electrophoresis
Disulfides
Detergents
Urea
Chorionic Villi
Glycoproteins
Scanning Transmission Electron Microscopy
Gels
Tissue
Transmission electron microscopy
Placenta

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ohno, M., Nagle, R. B., Meezan, E., & Brendel, K. (1979). Isolation and characterization of human placental chorionic villar extracellular matrix. In Journal of Supramolecular and Cellular Biochemistry (4 ed., Vol. 12, pp. 457-466)

Isolation and characterization of human placental chorionic villar extracellular matrix. / Ohno, M.; Nagle, Raymond B; Meezan, E.; Brendel, K.

Journal of Supramolecular and Cellular Biochemistry. Vol. 12 4. ed. 1979. p. 457-466.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ohno, M, Nagle, RB, Meezan, E & Brendel, K 1979, Isolation and characterization of human placental chorionic villar extracellular matrix. in Journal of Supramolecular and Cellular Biochemistry. 4 edn, vol. 12, pp. 457-466.
Ohno M, Nagle RB, Meezan E, Brendel K. Isolation and characterization of human placental chorionic villar extracellular matrix. In Journal of Supramolecular and Cellular Biochemistry. 4 ed. Vol. 12. 1979. p. 457-466
Ohno, M. ; Nagle, Raymond B ; Meezan, E. ; Brendel, K. / Isolation and characterization of human placental chorionic villar extracellular matrix. Journal of Supramolecular and Cellular Biochemistry. Vol. 12 4. ed. 1979. pp. 457-466
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