Isolation and characterization of mosquito ferritin and cloning of a cDNA that encodes one subunit

Boris C. Dunkov, Dianzheng Zhang, Kyril Choumarov, Joy J. Winzerling, John H. Law

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Ferritin, an iron storage protein, was isolated from larvae and pupae of Aedes aegypti grown in an iron‐rich medium. Mosquito ferritin is a high molecular weight protein composed of several different, relatively small, subunits. Subunits of molecular mass 24, 26, and 28 kDa are equally abundant, while that of 30 kDa is present only in small amounts. The N‐terminal sequence of the 24 and 26 kDa subunits are identical for the first 30 amino acids, while that of the 28 kDa subunit differs. Studies using antiserum raised against a subunit mixture showed that the ferritin subunits were present in larvae, pupae, and adult females, and were increased in animals exposed to excess iron. The antiserum also was used to screen a cDNA library from unfed adult female mosquitoes. Nine clones were obtained that differed only in a 27 bp insertion in the 3′ end. Rapid amplification of cDNA ends (RACE) was used to obtain the complete protein coding sequence. A putative iron‐responsive element (IRE) is present in the 5′‐untranslated region. The deduced amino acid sequence shows a typical leader sequence, consistent with the fact that most insect ferritins are secreted, rather than cytoplasmic proteins. The sequence encodes a mature polypeptide of 20,566 molecular weight, smaller than the estimated size of any of the subunits. However, the sequence exactly matches the N‐terminal sequences of the 24 and 26 kDa subunits as determined by Edman degradation. Of the known ferritin sequences, that of the mosquito is most similar to that of somatic cells of a snail. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)293-307
Number of pages15
JournalArchives of insect biochemistry and physiology
Volume29
Issue number3
DOIs
StatePublished - 1995

Keywords

  • Aedes aegypti
  • cDNA sequence
  • cloning
  • expression
  • ferritin
  • mosquito

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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