Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse

Nathan J. Weyand, Mancheong Ma, Megan Phifer-Rixey, Nyiawung A. Taku, María A. Rendón, Alyson M. Hockenberry, Won J. Kim, Al B. Agellon, Nicolas Biais, Taichi A. Suzuki, Lily Goodyer-Sait, Odile B. Harrison, Holly B. Bratcher, Michael W. Nachman, Martin C J Maiden, Magdalene "Maggie" So

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria–host interactions.

Original languageEnglish (US)
Article number001237
Pages (from-to)3585-3593
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Neisseria
Mus musculus
felid
DNA
gene
new species
pig
primate
rodent
genomics
cavity
rRNA Operon
Felidae
animal
operon
genetic engineering
guinea pigs
Primates
internal transcribed spacers
Genes

Keywords

  • DNA uptake sequence
  • Genetic competence
  • Mus musculus domesticus
  • Neisseria
  • Type IV pilus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse. / Weyand, Nathan J.; Ma, Mancheong; Phifer-Rixey, Megan; Taku, Nyiawung A.; Rendón, María A.; Hockenberry, Alyson M.; Kim, Won J.; Agellon, Al B.; Biais, Nicolas; Suzuki, Taichi A.; Goodyer-Sait, Lily; Harrison, Odile B.; Bratcher, Holly B.; Nachman, Michael W.; Maiden, Martin C J; So, Magdalene "Maggie".

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 9, 001237, 01.09.2016, p. 3585-3593.

Research output: Contribution to journalArticle

Weyand, NJ, Ma, M, Phifer-Rixey, M, Taku, NA, Rendón, MA, Hockenberry, AM, Kim, WJ, Agellon, AB, Biais, N, Suzuki, TA, Goodyer-Sait, L, Harrison, OB, Bratcher, HB, Nachman, MW, Maiden, MCJ & So, MM 2016, 'Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse', International Journal of Systematic and Evolutionary Microbiology, vol. 66, no. 9, 001237, pp. 3585-3593. https://doi.org/10.1099/ijsem.0.001237
Weyand, Nathan J. ; Ma, Mancheong ; Phifer-Rixey, Megan ; Taku, Nyiawung A. ; Rendón, María A. ; Hockenberry, Alyson M. ; Kim, Won J. ; Agellon, Al B. ; Biais, Nicolas ; Suzuki, Taichi A. ; Goodyer-Sait, Lily ; Harrison, Odile B. ; Bratcher, Holly B. ; Nachman, Michael W. ; Maiden, Martin C J ; So, Magdalene "Maggie". / Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 9. pp. 3585-3593.
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