Isolation and embryonic expression of an abdominal-A-like gene from the lepidopteran, Manduca sexta

Lisa M. Nagy, Ronald Booker, Lynn M. Riddiford

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Using sequence homology to the Drosophila Antennapedia gene, we isolated a homeobox-containing gene from the lepidopteran, Manduca sexta. Sequence analysis and in situ hybridizations to tissue sections suggest that the Manduca gene encodes a lepidopteran homologue of the Drosophila Bithorax complex gene abdominal-A. The predicted amino acid sequence of a 76 amino acid region that includes the homeobox and the regions immediately flanking it are identical between the Manduca and Drosophila genes. Northern blots reveal that the Manduca abd-A gene is expressed first in the early embryo and continues to be expressed throughout later embryonic and larval stages. In situ hybridizations show that the posterior half of the first abdominal segment marks the anterior border of the Manduca abd-A expression. This expression pattern demonstrates the conservation of parasegments as domains of gene activity in the lepidopteran embryo. The Manduca abd-A expression extends from the posterior half of the first abdominal segment through the tenth abdominal segment, a domain that is greater than that of the Drosophila abd-A expression, and reflects the difference in visible segment number between the two insects.

Original languageEnglish (US)
Pages (from-to)119-129
Number of pages11
JournalDevelopment
Volume112
Issue number1
StatePublished - May 1991
Externally publishedYes

Keywords

  • Abdominal-A
  • Homeobox-gene
  • Manduca sexta
  • Segmentation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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