Isolation and expression of an anther-specific gene from tomato

David Twell, Rod A Wing, Judy Yamaguchi, Sheila McCormick

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

We have isolated and sequenced an anther-specific cDNA clone and a corresponding genomic clone from tomato. The gene (LAT52) encodes an 800-nucleotide-long transcript that is detectable in pollen, anthers and at 20-to 50-fold lower levels in petals. LAT52 mRNA is not detectable in pistils, sepals or non-reproductive tissues. Steady-state levels of LAT52 mRNA are detectable in immature anthers containing pollen at the tetrad stage and increase progressively throughout microsporogenesis until anthesis (pollen shed). The LAT52 gene contains 5′ and 3′ untranslated regions of 110 and approximately 150 nucleotides, respectively, and a single intron with a highly repetitive sequence. A TATA box motif is located 28 nucleotides upstream of the transcription start site. The gene encodes a putative protein of 18 kDa that is cysteine rich and has an N-terminal hydrophobic region with characteristics similar to eucaryotic secretory signal sequences. LAT52 is a single or low copy gene in tomato and shares homology with sequences in tobacco.

Original languageEnglish (US)
Pages (from-to)240-245
Number of pages6
JournalMGG Molecular & General Genetics
Volume217
Issue number2-3
DOIs
StatePublished - Jun 1989
Externally publishedYes

Fingerprint

Lycopersicon esculentum
Pollen
Nucleotides
Genes
Plant Gametogenesis
Clone Cells
Messenger RNA
TATA Box
Nucleic Acid Repetitive Sequences
5' Untranslated Regions
Transcription Initiation Site
3' Untranslated Regions
Sequence Homology
Protein Sorting Signals
Introns
Tobacco
Cysteine
Complementary DNA
Proteins

Keywords

  • Anther
  • Lycopersicon esculentum
  • Microsporogenesis
  • Pollen

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation and expression of an anther-specific gene from tomato. / Twell, David; Wing, Rod A; Yamaguchi, Judy; McCormick, Sheila.

In: MGG Molecular & General Genetics, Vol. 217, No. 2-3, 06.1989, p. 240-245.

Research output: Contribution to journalArticle

Twell, David ; Wing, Rod A ; Yamaguchi, Judy ; McCormick, Sheila. / Isolation and expression of an anther-specific gene from tomato. In: MGG Molecular & General Genetics. 1989 ; Vol. 217, No. 2-3. pp. 240-245.
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