Isolation and identification of entomopathogenic nematodes and their symbiotic bacteria from Hérault and Gard (Southern France)

Vanya Emelianoff, Nathalie Le Brun, Sylvie Pagès, S Patricia Stock, Patrick Tailliez, Catherine Moulia, Mathieu Sicard

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Isolation and identification of native nematode-bacterial associations in the field are necessary for successful control of endemic pests in a particular location. No study has yet been undertaken to recover and identify EPN in metropolitan France. In the present paper, we provide results of a survey of EPN and their symbiotic bacteria conducted in Hérault and Gard regions in Southern France. Molecular characterization of isolated nematodes depicted three different Steinernema species and one Heterorhabditis species, H. bacteriophora. Steinernema species recovered were identified as: S. feltiae and S. affine and an undescribed species. Xenorhabdus symbionts were identified as X. bovienii for both S. feltiae and S. affine. Phylogenetic analysis placed the new undescribed Steinernema sp. as closely related to S. arenarium but divergent enough to postulate that it belongs to a new species within the "glaseri-group". The Xenorhabdus symbiont from this Steinernema sp. was identified as X. kozodoii. All Heterorhabditis isolates recovered were diagnosed as H. bacteriophora and their bacterial symbionts were identified as Photorhabdus luminescens. Molecular characterization of these nematodes enabled the distinction of two different H. bacteriophora strains. Bacterial symbiontic strains of these two H. bacteriophora strains were identified as P. luminescens ssp. kayaii and P. luminescens ssp. laumondii.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Invertebrate Pathology
Volume98
Issue number2
DOIs
StatePublished - Jun 2008

Fingerprint

Steinernema
entomopathogenic nematodes
Photorhabdus luminescens
nematode
symbiont
France
Xenorhabdus
symbionts
Heterorhabditis
Heterorhabditis bacteriophora
bacterium
bacteria
Nematoda
Xenorhabdus bovienii
Steinernema feltiae
pests
new species
phylogenetics
phylogeny

Keywords

  • Entomopathogenic nematodes
  • Heterorhabditis
  • Host-symbiont specificity
  • Molecular phylogeny
  • Photorhabdus
  • Steinernema
  • Symbiosis
  • Xenorhabdus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Isolation and identification of entomopathogenic nematodes and their symbiotic bacteria from Hérault and Gard (Southern France). / Emelianoff, Vanya; Le Brun, Nathalie; Pagès, Sylvie; Stock, S Patricia; Tailliez, Patrick; Moulia, Catherine; Sicard, Mathieu.

In: Journal of Invertebrate Pathology, Vol. 98, No. 2, 06.2008, p. 211-217.

Research output: Contribution to journalArticle

Emelianoff, Vanya ; Le Brun, Nathalie ; Pagès, Sylvie ; Stock, S Patricia ; Tailliez, Patrick ; Moulia, Catherine ; Sicard, Mathieu. / Isolation and identification of entomopathogenic nematodes and their symbiotic bacteria from Hérault and Gard (Southern France). In: Journal of Invertebrate Pathology. 2008 ; Vol. 98, No. 2. pp. 211-217.
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