Abstract
Many important cellular processes are mediated by sequence-specific RNA binding proteins, and it is often necessary to purify these proteins. When the RNA binding site is known, it is convenient to use this RNA as a matrix for affinity purification. The intronic splicing silencer (ISS) element present upstream of the N1 exon of the c-src pre-mRNA is a high-affinity binding site for the polypyrimidine tract binding protein (PTB). Using a 5'-biotinylated ISS RNA and PTB as an example, I describe a one-step method for affinity chromatography of RNA binding proteins from nuclear extracts.
Original language | English (US) |
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Pages (from-to) | 1-8 |
Number of pages | 8 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 488 |
State | Published - 2008 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Genetics