Isolation of circulating tumor cells under hydrodynamic loading using microuidic technology

Cong Zhao, Yi Kuen Lee, Rui Xu, Chun Liang, Dayu Liu, Wei Ma, Wibool Piyawattanametha, Yitshak Zohar

Research output: Contribution to journalArticle

Abstract

Cancer is a leading cause of mortality worldwide causing human deaths. Circulating tumor cells (CTCs) are cells that have detached from a primary tumor and circulate in the bloodstream; they may constitute seeds for subsequent growth of additional tumors (metastasis) in different tissues. The detection of CTCs may have important prognostic and therapeutic implications but, because their number is very small, these cells are not easily detected. Circulating tumor cells are found in the order of 10-100 CTCs per mL of whole blood in patients with metastatic disease. Isolation of tumor cells circulating in the blood stream, by immobilizing them on surfaces functionalized with bio-active coating within microfluidic devices, presents an interdisciplinary challenge requiring expertise in different research areas: cell biology, surface chemistry, fluid mechanics and microsystem technology. We first review the fundamental of cell biology of CTCs and summarize the key microfluidic techniques for isolation of CTCs via cell-ligand interactions, magnetic interactions, filtration; detection and enumeration of CTCs; in vivo CTCs imaging.

Original languageEnglish (US)
Pages (from-to)447-494
Number of pages48
JournalAdvances in Mechanics
Volume44
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • Cancer
  • Circulating tumor cells (CTC)
  • Epithelial-mesenchymal transitions
  • Metastasis
  • Micro-electro mechanical system (MEMS)
  • Microfluidics
  • Microsystem

ASJC Scopus subject areas

  • Mathematical Physics
  • Mechanical Engineering

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