Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor

N. B. Egen, M. Bliss, Michael Mayersohn, S. M. Owens, L. Arnold, M. Bier

Research output: Contribution to journalArticle

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Abstract

A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.

Original languageEnglish (US)
Pages (from-to)488-494
Number of pages7
JournalAnalytical Biochemistry
Volume172
Issue number2
DOIs
StatePublished - Aug 1 1988

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Phencyclidine
Isoelectric Focusing
Ascites
Monoclonal Antibodies
Fluids
Dialysis
Antibodies
Purification

Keywords

  • ascites fluid
  • isoelectric focusing
  • monoclonal antibody
  • phencyclidine
  • purification
  • Rotofor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor. / Egen, N. B.; Bliss, M.; Mayersohn, Michael; Owens, S. M.; Arnold, L.; Bier, M.

In: Analytical Biochemistry, Vol. 172, No. 2, 01.08.1988, p. 488-494.

Research output: Contribution to journalArticle

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