Isolation of the promoter of a root cap expressed pectinmethylesterase gene from Pisum sativum L. (rcpme1) and its use in the study of gene activity

Y. Zhu, F. Wen, X. Zhao, M. C. Hawes

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A genomic clone of a pea pectinmethylesterase encoding gene, rcpme1, was isolated; the promoter region was found to include regions of homology to phenylalanine ammonia lyase (PAL) and nodulin gene promoters. Agrobacterium rhizogenes mediated hairy roots were used for rcpme1 expression and functional analysis in pea. Patterns of rcpme1 expression in cultured hairy roots, measured using uidA encoding β-glucuronidase (GUS) as a reporter gene, were distinct from patterns which occur in normal pea roots. No reporter gene expression occurred in transgenic Arabidopsis thaliana, whose roots do not produce border cells. Border cell number from transgenic hairy roots expressing rcpme1 anti-sense mRNA under the control of its 2.75 kb 5prime; flanking sequence was reduced by > 50%. Nodulation genes of Rhizobium leguminosarum were used as a marker to document that roots with reduced production of border cells and other root cap exudates have a corresponding reduction in levels of biologically active signal molecules. Direct measurements were used to confirm that most of the exudate harvested from young, unwounded roots of normal pea plants is derived from the root tip region where rcpme1 is expressed. The potential application of the rcpme1 gene as a molecular marker for root exudate production is discussed.

Original languageEnglish (US)
Pages (from-to)47-59
Number of pages13
JournalPlant and Soil
Volume265
Issue number1-2
DOIs
StatePublished - Aug 1 2004

Keywords

  • Agrobacterium rhizogenes
  • Cell wall degrading enzymes
  • Hairy roots
  • Root border cells

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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