K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose

Andre I. Sarabia-Sainz, Andre I. Sarabia-Sainz, Hector Manuel Sarabia-Sainz, Gabriela Ramos Clamont Montfort, Veronica Mata-Haro, Ana María Guzman-Partida, Roberto Z Guzman, Mariano Garcia-Soto, Luz Vazquez-Moreno

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10–17 μm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections.

Original languageEnglish (US)
Pages (from-to)22425-22437
Number of pages13
JournalInternational Journal of Molecular Sciences
Volume16
Issue number9
DOIs
StatePublished - Sep 16 2015

Keywords

  • Gentamicin
  • Lactosylated microspheres
  • Targeted active drug

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

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  • Cite this

    Sarabia-Sainz, A. I., Sarabia-Sainz, A. I., Sarabia-Sainz, H. M., Montfort, G. R. C., Mata-Haro, V., Guzman-Partida, A. M., Guzman, R. Z., Garcia-Soto, M., & Vazquez-Moreno, L. (2015). K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose. International Journal of Molecular Sciences, 16(9), 22425-22437. https://doi.org/10.3390/ijms160922425