K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose

Andre I. Sarabia-Sainz, Andre I. Sarabia-Sainz, Hector Manuel Sarabia-Sainz, Gabriela Ramos Clamont Montfort, Veronica Mata-Haro, Ana María Guzman-Partida, Roberto Z Guzman, Mariano Garcia-Soto, Luz Vazquez-Moreno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10–17 μm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections.

Original languageEnglish (US)
Pages (from-to)22425-22437
Number of pages13
JournalInternational Journal of Molecular Sciences
Volume16
Issue number9
DOIs
StatePublished - Sep 16 2015

Fingerprint

Bacterial Adhesins
lactose
Lactose
Escherichia
Gentamicins
Microspheres
albumins
galactose
Escherichia coli
Escherichia coli Adhesins
Galactose
delivery
antibiotics
infectious diseases
Antibiotics
crosslinking
Sophora
glucose
Crosslinking
Electron microscopy

Keywords

  • Gentamicin
  • Lactosylated microspheres
  • Targeted active drug

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

Cite this

Sarabia-Sainz, A. I., Sarabia-Sainz, A. I., Sarabia-Sainz, H. M., Montfort, G. R. C., Mata-Haro, V., Guzman-Partida, A. M., ... Vazquez-Moreno, L. (2015). K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose. International Journal of Molecular Sciences, 16(9), 22425-22437. https://doi.org/10.3390/ijms160922425

K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose. / Sarabia-Sainz, Andre I.; Sarabia-Sainz, Andre I.; Sarabia-Sainz, Hector Manuel; Montfort, Gabriela Ramos Clamont; Mata-Haro, Veronica; Guzman-Partida, Ana María; Guzman, Roberto Z; Garcia-Soto, Mariano; Vazquez-Moreno, Luz.

In: International Journal of Molecular Sciences, Vol. 16, No. 9, 16.09.2015, p. 22425-22437.

Research output: Contribution to journalArticle

Sarabia-Sainz, AI, Sarabia-Sainz, AI, Sarabia-Sainz, HM, Montfort, GRC, Mata-Haro, V, Guzman-Partida, AM, Guzman, RZ, Garcia-Soto, M & Vazquez-Moreno, L 2015, 'K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose', International Journal of Molecular Sciences, vol. 16, no. 9, pp. 22425-22437. https://doi.org/10.3390/ijms160922425
Sarabia-Sainz AI, Sarabia-Sainz AI, Sarabia-Sainz HM, Montfort GRC, Mata-Haro V, Guzman-Partida AM et al. K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose. International Journal of Molecular Sciences. 2015 Sep 16;16(9):22425-22437. https://doi.org/10.3390/ijms160922425
Sarabia-Sainz, Andre I. ; Sarabia-Sainz, Andre I. ; Sarabia-Sainz, Hector Manuel ; Montfort, Gabriela Ramos Clamont ; Mata-Haro, Veronica ; Guzman-Partida, Ana María ; Guzman, Roberto Z ; Garcia-Soto, Mariano ; Vazquez-Moreno, Luz. / K88 fimbrial adhesin targeting of microspheres containing gentamicin made with albumin glycated with lactose. In: International Journal of Molecular Sciences. 2015 ; Vol. 16, No. 9. pp. 22425-22437.
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