Kappa opioid signaling in the central nucleus of the amygdala promotes disinhibition and aversiveness of chronic neuropathic pain

Edita Navratilova, Guangchen Ji, Caroline Phelps, Chaoling Qu, Matthew Hein, Vadim Yakhnitsa, Volker Neugebauer, Frank Porreca

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chronic pain is associated with neuroplastic changes in the amygdala that may promote hyper-responsiveness to mechanical and thermal stimuli (allodynia and hyperalgesia) and/or enhance emotional and affective consequences of pain. Stress promotes dynorphin-mediated signaling at the kappa opioid receptor (KOR) in the amygdala and mechanical hypersensitivity in rodent models of functional pain. Here, we tested the hypothesis that KOR circuits in the central nucleus of the amygdala (CeA) undergo neuroplasticity in chronic neuropathic pain resulting in increased sensory and affective pain responses. After spinal nerve ligation (SNL) injury in rats, pretreatment with a long-acting KOR antagonist, nor-binaltorphimine (nor-BNI), subcutaneously or through microinjection into the right CeA, prevented conditioned place preference (CPP) to intravenous gabapentin, suggesting that nor-BNI eliminated the aversiveness of ongoing pain. By contrast, systemic or intra-CeA administration of nor-BNI had no effect on tactile allodynia in SNL animals. Using whole-cell patch-clamp electrophysiology, we found that nor-BNI decreased synaptically evoked spiking of CeA neurons in brain slices from SNL but not sham rats. This effect was mediated through increased inhibitory postsynaptic currents, suggesting tonic disinhibition of CeA output neurons due to increased KOR activity as a possible mechanism promoting ongoing aversive aspects of neuropathic pain. Interestingly, this mechanism is not involved in SNL-induced mechanical allodynia. Kappa opioid receptor antagonists may therefore represent novel therapies for neuropathic pain by targeting aversive aspects of ongoing pain while preserving protective functions of acute pain.

Original languageEnglish (US)
Pages (from-to)824-832
Number of pages9
JournalPain
Volume160
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

kappa Opioid Receptor
Neuralgia
Spinal Nerves
Chronic Pain
Opioid Analgesics
Hyperalgesia
Ligation
Pain
Narcotic Antagonists
Amygdala
Neurons
Dynorphins
Inhibitory Postsynaptic Potentials
Neuronal Plasticity
Electrophysiology
Acute Pain
Microinjections
Rodentia
Hypersensitivity
Central Amygdaloid Nucleus

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Kappa opioid signaling in the central nucleus of the amygdala promotes disinhibition and aversiveness of chronic neuropathic pain. / Navratilova, Edita; Ji, Guangchen; Phelps, Caroline; Qu, Chaoling; Hein, Matthew; Yakhnitsa, Vadim; Neugebauer, Volker; Porreca, Frank.

In: Pain, Vol. 160, No. 4, 01.04.2019, p. 824-832.

Research output: Contribution to journalArticle

Navratilova, Edita ; Ji, Guangchen ; Phelps, Caroline ; Qu, Chaoling ; Hein, Matthew ; Yakhnitsa, Vadim ; Neugebauer, Volker ; Porreca, Frank. / Kappa opioid signaling in the central nucleus of the amygdala promotes disinhibition and aversiveness of chronic neuropathic pain. In: Pain. 2019 ; Vol. 160, No. 4. pp. 824-832.
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