Karyometry of breast epithelial cells acquired by random periareolar fine needle aspiration in women at high risk for breast cancer

Peter H. Bartels, Carol J. Fabian, Bruce F. Kimler, James R. Ranger-Moore, Denise H Frank, Michael L. Yozwiak, David S Alberts

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

OBJECTIVE: To establish whether karyometry was likely to detect change in the proportion of abnormal cells in random periareolar fine needle aspiration (RPFNA) specimens from high-risk women in a 6-month prevention trial with an aromatase inhibitor. STUDY DESIGN: Papanicolaou-stained ThinPrep slides of RPFNA samples from 11 of 42 women were digitally recorded at high resolution, with 200 cells measured per slide, at baseline (BL) and at the end of study (ES) after 6 months. The nuclear chromatin pattern characteristics were assessed by multivariate analytic techniques; determination of nuclear abnormalities was performed and cells that showed expression of abnormality were identified. RESULTS: The BL FNA samples contain ∼90% cells with a chromatin pattern as expected in a normal cell population. A small subpopulation of cells had deviations from normal. At ES the proportion of these cells was reduced, to a statistically significant degree, from < 10% to 2-5%. CONCLUSION: Nuclear karyometry is a promising technique for characterizing the proportion of cells deviating from normal in cytologic specimens and should be explored further as an intermediate endpoint in prevention trials.

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalAnalytical and Quantitative Cytology and Histology
Volume29
Issue number2
StatePublished - Apr 2007

Fingerprint

Karyometry
Fine Needle Biopsy
Breast
Epithelial Cells
Breast Neoplasms
Chromatin
Aromatase Inhibitors

Keywords

  • Aspiration cytology, fine needle
  • Chemoprevention
  • Karyometry
  • Letrozole

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Cell Biology

Cite this

Karyometry of breast epithelial cells acquired by random periareolar fine needle aspiration in women at high risk for breast cancer. / Bartels, Peter H.; Fabian, Carol J.; Kimler, Bruce F.; Ranger-Moore, James R.; Frank, Denise H; Yozwiak, Michael L.; Alberts, David S.

In: Analytical and Quantitative Cytology and Histology, Vol. 29, No. 2, 04.2007, p. 63-70.

Research output: Contribution to journalArticle

Bartels, Peter H. ; Fabian, Carol J. ; Kimler, Bruce F. ; Ranger-Moore, James R. ; Frank, Denise H ; Yozwiak, Michael L. ; Alberts, David S. / Karyometry of breast epithelial cells acquired by random periareolar fine needle aspiration in women at high risk for breast cancer. In: Analytical and Quantitative Cytology and Histology. 2007 ; Vol. 29, No. 2. pp. 63-70.
@article{8d96c812fc7d4d179c09b5e6013fb1af,
title = "Karyometry of breast epithelial cells acquired by random periareolar fine needle aspiration in women at high risk for breast cancer",
abstract = "OBJECTIVE: To establish whether karyometry was likely to detect change in the proportion of abnormal cells in random periareolar fine needle aspiration (RPFNA) specimens from high-risk women in a 6-month prevention trial with an aromatase inhibitor. STUDY DESIGN: Papanicolaou-stained ThinPrep slides of RPFNA samples from 11 of 42 women were digitally recorded at high resolution, with 200 cells measured per slide, at baseline (BL) and at the end of study (ES) after 6 months. The nuclear chromatin pattern characteristics were assessed by multivariate analytic techniques; determination of nuclear abnormalities was performed and cells that showed expression of abnormality were identified. RESULTS: The BL FNA samples contain ∼90{\%} cells with a chromatin pattern as expected in a normal cell population. A small subpopulation of cells had deviations from normal. At ES the proportion of these cells was reduced, to a statistically significant degree, from < 10{\%} to 2-5{\%}. CONCLUSION: Nuclear karyometry is a promising technique for characterizing the proportion of cells deviating from normal in cytologic specimens and should be explored further as an intermediate endpoint in prevention trials.",
keywords = "Aspiration cytology, fine needle, Chemoprevention, Karyometry, Letrozole",
author = "Bartels, {Peter H.} and Fabian, {Carol J.} and Kimler, {Bruce F.} and Ranger-Moore, {James R.} and Frank, {Denise H} and Yozwiak, {Michael L.} and Alberts, {David S}",
year = "2007",
month = "4",
language = "English (US)",
volume = "29",
pages = "63--70",
journal = "Analytical and Quantitative Cytopathology and Histopathology",
issn = "0301-102X",
publisher = "John Rylands University Library",
number = "2",

}

TY - JOUR

T1 - Karyometry of breast epithelial cells acquired by random periareolar fine needle aspiration in women at high risk for breast cancer

AU - Bartels, Peter H.

AU - Fabian, Carol J.

AU - Kimler, Bruce F.

AU - Ranger-Moore, James R.

AU - Frank, Denise H

AU - Yozwiak, Michael L.

AU - Alberts, David S

PY - 2007/4

Y1 - 2007/4

N2 - OBJECTIVE: To establish whether karyometry was likely to detect change in the proportion of abnormal cells in random periareolar fine needle aspiration (RPFNA) specimens from high-risk women in a 6-month prevention trial with an aromatase inhibitor. STUDY DESIGN: Papanicolaou-stained ThinPrep slides of RPFNA samples from 11 of 42 women were digitally recorded at high resolution, with 200 cells measured per slide, at baseline (BL) and at the end of study (ES) after 6 months. The nuclear chromatin pattern characteristics were assessed by multivariate analytic techniques; determination of nuclear abnormalities was performed and cells that showed expression of abnormality were identified. RESULTS: The BL FNA samples contain ∼90% cells with a chromatin pattern as expected in a normal cell population. A small subpopulation of cells had deviations from normal. At ES the proportion of these cells was reduced, to a statistically significant degree, from < 10% to 2-5%. CONCLUSION: Nuclear karyometry is a promising technique for characterizing the proportion of cells deviating from normal in cytologic specimens and should be explored further as an intermediate endpoint in prevention trials.

AB - OBJECTIVE: To establish whether karyometry was likely to detect change in the proportion of abnormal cells in random periareolar fine needle aspiration (RPFNA) specimens from high-risk women in a 6-month prevention trial with an aromatase inhibitor. STUDY DESIGN: Papanicolaou-stained ThinPrep slides of RPFNA samples from 11 of 42 women were digitally recorded at high resolution, with 200 cells measured per slide, at baseline (BL) and at the end of study (ES) after 6 months. The nuclear chromatin pattern characteristics were assessed by multivariate analytic techniques; determination of nuclear abnormalities was performed and cells that showed expression of abnormality were identified. RESULTS: The BL FNA samples contain ∼90% cells with a chromatin pattern as expected in a normal cell population. A small subpopulation of cells had deviations from normal. At ES the proportion of these cells was reduced, to a statistically significant degree, from < 10% to 2-5%. CONCLUSION: Nuclear karyometry is a promising technique for characterizing the proportion of cells deviating from normal in cytologic specimens and should be explored further as an intermediate endpoint in prevention trials.

KW - Aspiration cytology, fine needle

KW - Chemoprevention

KW - Karyometry

KW - Letrozole

UR - http://www.scopus.com/inward/record.url?scp=34247505030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247505030&partnerID=8YFLogxK

M3 - Article

C2 - 17484269

AN - SCOPUS:34247505030

VL - 29

SP - 63

EP - 70

JO - Analytical and Quantitative Cytopathology and Histopathology

JF - Analytical and Quantitative Cytopathology and Histopathology

SN - 0301-102X

IS - 2

ER -