Kinematics of specifically captured circulating tumor cells in bio-functionalized microchannels

Luthur Siu Lun Cheung, Xiangjun Zheng, Lian Wang, Roberto Guzman, Joyce A. Schroeder, Ronald L. Heimark, James C. Baygents, Yitshak Zohar

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The attachment kinematics of cancer cells under hydrodynamic loading in antibody-functionalized microchannels has been studied. Epithelial-cell- adhesion-molecule antibodies are immobilized on the microchannel surface for specific capture of the target cancer cells from homogeneous cell suspensions. The specific interaction between the cancer cell receptors and the immobilized antibodies under static conditions is demonstrated. The capture efficiency of the target cells from homogeneous suspensions under applied hydrodynamic flow field has been investigated, revealing a characteristic shear stress. Applying a lower stress allows the capture of most target cells, while the capture efficiency drops sharply with an increasing shear stress. The captured cells are spatially distributed along the microchannel; both the velocity and the distance travelled by cells prior to capture are measured. The characteristic time and length scales for cell capture are determined, and a log-normal statistical distribution is proposed to describe the observations. Furthermore, a first-order kinetic model for receptorligand bond formation provides a rough estimate of the cell adhesion rate constant. Under a low shear stress, the on-rate is much higher than the off-rate, allowing capture of most loaded cells. The off-rate constant increases exponentially with an increasing shear stress, such that above the characteristic stress level, most loaded cells avoid capture.

Original languageEnglish (US)
Article number5497059
Pages (from-to)752-763
Number of pages12
JournalJournal of Microelectromechanical Systems
Volume19
Issue number4
DOIs
StatePublished - Aug 1 2010

Keywords

  • Bio-functional microchannels
  • cell-capture kinetics
  • circulating tumor cells (CTCs)

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering

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