Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay

D. P. Henry, David G Johnson, B. J. Starman, R. H. Williams

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.

Original languageEnglish (US)
Pages (from-to)1179-1186
Number of pages8
JournalLife Sciences
Volume17
Issue number7
DOIs
StatePublished - Oct 1 1975
Externally publishedYes

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Dopamine beta-Hydroxylase
Rats
Assays
Kinetics
Serum
Ions
S-Adenosylmethionine
Fumarates
Tyramine
Transferases
Ascorbic Acid
Ethanol

ASJC Scopus subject areas

  • Pharmacology

Cite this

Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay. / Henry, D. P.; Johnson, David G; Starman, B. J.; Williams, R. H.

In: Life Sciences, Vol. 17, No. 7, 01.10.1975, p. 1179-1186.

Research output: Contribution to journalArticle

Henry, D. P. ; Johnson, David G ; Starman, B. J. ; Williams, R. H. / Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay. In: Life Sciences. 1975 ; Vol. 17, No. 7. pp. 1179-1186.
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