Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay

D. P. Henry; D. G. Johnson; B. J. Starman; R. H. Williams

doi:10.1016/0024-3205(75)90341-0

Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay

D. P. Henry, D. G. Johnson, B. J. Starman, R. H. Williams

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and ³(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.

Original language	English (US)
Pages (from-to)	1179-1186
Number of pages	8
Journal	Life Sciences
Volume	17
Issue number	7
DOIs	https://doi.org/10.1016/0024-3205(75)90341-0
State	Published - Oct 1 1975
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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title = "Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay",

abstract = "A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.",

author = "Henry, {D. P.} and Johnson, {D. G.} and Starman, {B. J.} and Williams, {R. H.}",

note = "Funding Information: This research was supported by NIH research grants AM 05020-19 and AM 02456 .. D .P . Henrv was a NIH Special Research Follow (F03 AM 53427) and D .G . Johnson in the recipient of a NIH Career Development Award (KO4 AM 70727) .",

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doi = "10.1016/0024-3205(75)90341-0",

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T1 - Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay

AU - Henry, D. P.

AU - Johnson, D. G.

AU - Starman, B. J.

AU - Williams, R. H.

N1 - Funding Information: This research was supported by NIH research grants AM 05020-19 and AM 02456 .. D .P . Henrv was a NIH Special Research Follow (F03 AM 53427) and D .G . Johnson in the recipient of a NIH Career Development Award (KO4 AM 70727) .

PY - 1975/10/1

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N2 - A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.

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Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay

Abstract

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