Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay

D. P. Henry, D. G. Johnson, B. J. Starman, R. H. Williams

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.

Original languageEnglish (US)
Pages (from-to)1179-1186
Number of pages8
JournalLife Sciences
Volume17
Issue number7
DOIs
StatePublished - Oct 1 1975
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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