Abstract
A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1179-1186 |
| Number of pages | 8 |
| Journal | Life Sciences |
| Volume | 17 |
| Issue number | 7 |
| DOIs | |
| State | Published - Oct 1 1975 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pharmacology
Cite this
Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay. / Henry, D. P.; Johnson, David G; Starman, B. J.; Williams, R. H.
In: Life Sciences, Vol. 17, No. 7, 01.10.1975, p. 1179-1186.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kinetic characterization of rat serum dopamine-beta-hydroxylase using a simplified radioenzymatic assay
AU - Henry, D. P.
AU - Johnson, David G
AU - Starman, B. J.
AU - Williams, R. H.
PY - 1975/10/1
Y1 - 1975/10/1
N2 - A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.
AB - A simplified radioenzymatic assay for dopamine-beta-hydroxylase (DBH), utilizing partially purified phenyl-ethanol-N-methyl-transferase and 3(H)-S-adenosylmethionine, was developed and used to characterize DBH activity found in rat serum. The Km of DBH for ascorbic acid was 1.34 mM. The Km for tyramine was a complex function of the concentration of the allosteric activator, fumarate, and the relative concentration of cupric ion compared to inhibitor concentration. The kinetics of the effect of cupric ion on DBH inhibitor were investigated extensively.
UR - http://www.scopus.com/inward/record.url?scp=0016695164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016695164&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(75)90341-0
DO - 10.1016/0024-3205(75)90341-0
M3 - Article
C2 - 573
AN - SCOPUS:0016695164
VL - 17
SP - 1179
EP - 1186
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 7
ER -