Kinetics and mechanism of dissimilative Fe(III) reduction by Pseudomonas sp. 200

Robert G. Arnold, Terese M. Olson, Michael R. Hoffmann

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The kinetics and mechanism of Fe(III) reduction to Fe(II) were studied in pure batch cultures of Pseudomonas sp. 200. The rate of iron reduction has been mechanistically related to aqueous phase iron speciation. In the absence of microbial activity the iron reduction rate was negligible. Initial rates of microbial iron reduction were accelerated more than 20‐fold by the addition of equimolar quantities of nitrilotriacetic acid (NTA) to media initially containing 1.86 × 10−3M total Fe(III). Numerical techniques were utilized to quantify relationships between the observed rate of Fe(II) production and the calculated (equilibrium) aqueous phase speciation. These results indicate that soluble ferric iron species are not equivalent in terms of their susceptibility to bacterial (dissimilative) iron reduction. The concentration of Fe(NTA)(OH) 22− correlated strongly with observed iron reduction rates. Ferrous iron species appeared to inhibit the reduction process.

Original languageEnglish (US)
Pages (from-to)1657-1671
Number of pages15
JournalBiotechnology and Bioengineering
Volume28
Issue number11
DOIs
StatePublished - Nov 1986

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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