Kinetics of sodium succinate cotransport across renal brush-border membranes

Stephen Wright, B. Hirayama, J. D. Kaunitz, I. Kippen, E. M. Wright

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The kinetics of Na/succinate cotransport across renal brush-borders was studied using membrane vesicles. Initial rates of succinate uptake (J(s)) were estimated from 1-s uptakes measured under voltage-clamped conditions. Lowering the external (cis) sodium concentration reduced the succinate K(t) without affecting the J(s)(max). Increasing the intravesicular (trans) sodium concentration reduced both J(s)(max) and K(t). This trans inhibition by Na was relieved by trans-succinate. Varying the membrane potential from -60 to +60 reduced succinate transport in an exponential manner, which was reflected by a K(t) effect, i.e. an increase in the K(t) with no change in J(s)(max). The membrane potential results suggest that the succinate/sodium-carrier complex bears a net positive charge. On the basis of these and earlier observations, we propose that Na/succinate cotransport across renal brush-borders is described by an Iso Ordered Quad Quad reaction kinetics in which three Na ions first bind to the carrier to increase its affinity for succinate.

Original languageEnglish (US)
Pages (from-to)5456-5462
Number of pages7
JournalJournal of Biological Chemistry
Volume258
Issue number9
StatePublished - 1983
Externally publishedYes

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Succinic Acid
Brushes
Microvilli
Sodium
Membranes
Kidney
Kinetics
Membrane Potentials
Reaction kinetics
Ions
Electric potential

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wright, S., Hirayama, B., Kaunitz, J. D., Kippen, I., & Wright, E. M. (1983). Kinetics of sodium succinate cotransport across renal brush-border membranes. Journal of Biological Chemistry, 258(9), 5456-5462.

Kinetics of sodium succinate cotransport across renal brush-border membranes. / Wright, Stephen; Hirayama, B.; Kaunitz, J. D.; Kippen, I.; Wright, E. M.

In: Journal of Biological Chemistry, Vol. 258, No. 9, 1983, p. 5456-5462.

Research output: Contribution to journalArticle

Wright, S, Hirayama, B, Kaunitz, JD, Kippen, I & Wright, EM 1983, 'Kinetics of sodium succinate cotransport across renal brush-border membranes', Journal of Biological Chemistry, vol. 258, no. 9, pp. 5456-5462.
Wright, Stephen ; Hirayama, B. ; Kaunitz, J. D. ; Kippen, I. ; Wright, E. M. / Kinetics of sodium succinate cotransport across renal brush-border membranes. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 9. pp. 5456-5462.
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