Lab-on-a-Chip for Field Escherichia coli Assays

Long-Term Stability of Reagents and Automatic Sampling System

Hyuck Jin Kwon, Zachary S. Dean, Scott V. Angus, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A field lab-on-a-chip system was constructed to detect mouse immunoglobulin G (mIgG; model protein target) and . Escherichia coli (. E. coli; model microorganism target) by using light scattering detection of particle immunoagglutination. The antibodies to these targets were conjugated to the submicron particles by covalent binding, and their long-term stability was evaluated. Antibody-conjugated particles were able to be stored in a 4. °C refrigerator for at least 4 weeks and to be lyophilized as a powder form for the storage in room temperature. The optimum antibody coverage on the particles was 50% for mIgG and 100% for . E. coli in terms of assay sensitivity and long-term storage of reagents. Lab-on-a-chip device was fabricated from acrylic plate using an industrial-grade milling machine eliminating the need for photolithography and internal or external pumping. An automatic sampling system was constructed using drip emitters, such that the system can be connected to a pressurized water pipe for detecting . E. coli. The automatic sampling system generated the same volume of droplets (70. μL) regardless of pressure. The developed system was successfully tested for . E. coli presence in field water samples. The system can potentially be connected to pressurized pipe networks for drinking, processing, irrigation, and wastewater.

Original languageEnglish (US)
Pages (from-to)216-223
Number of pages8
JournalJALA - Journal of the Association for Laboratory Automation
Volume15
Issue number3
DOIs
StatePublished - Jun 2010

Fingerprint

Lab-on-a-chip
Escherichia coli
Assays
Sampling
Antibodies
Pipe
Milling machines
Water
Refrigerators
Photolithography
Waste Water
Irrigation
Microorganisms
Light scattering
Powders
Drinking
Acrylics
Wastewater
Immunoglobulin G
Proteins

Keywords

  • Drip emitter
  • Microfluidic
  • Water distribution network
  • Waterborne pathogen

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Computer Science Applications

Cite this

Lab-on-a-Chip for Field Escherichia coli Assays : Long-Term Stability of Reagents and Automatic Sampling System. / Kwon, Hyuck Jin; Dean, Zachary S.; Angus, Scott V.; Yoon, Jeong-Yeol.

In: JALA - Journal of the Association for Laboratory Automation, Vol. 15, No. 3, 06.2010, p. 216-223.

Research output: Contribution to journalArticle

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