Lab-on-a-Chip for Field Escherichia Coli Assays: Long-Term Stability of Reagents and Automatic Sampling System

Hyuck Jin Kwon, Zachary S. Dean, Scott V. Angus, Jeong Yeol Yoon

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A field lab-on-a-chip system was constructed to detect mouse immunoglobulin G (mIgG; model protein target) and Escherichia coli (E. coli; model microorganism target) by using light scattering detection of particle immunoagglutination. The antibodies to these targets were conjugated to the submicron particles by covalent binding, and their long-term stability was evaluated. Antibody-conjugated particles were able to be stored in a 4 °C refrigerator for at least 4 weeks and to be lyophilized as a powder form for the storage in room temperature. The optimum antibody coverage on the particles was 50% for mIgG and 100% for E. coli in terms of assay sensitivity and long-term storage of reagents. Lab-on-a-chip device was fabricated from acrylic plate using an industrial-grade milling machine eliminating the need for photolithography and internal or external pumping. An automatic sampling system was constructed using drip emitters, such that the system can be connected to a pressurized water pipe for detecting E. coli. The automatic sampling system generated the same volume of droplets (70 μL) regardless of pressure. The developed system was successfully tested for E. coli presence in field water samples. The system can potentially be connected to pressurized pipe networks for drinking, processing, irrigation, and wastewater.

Original languageEnglish (US)
Pages (from-to)216-223
Number of pages8
JournalJournal of Laboratory Automation
Volume15
Issue number3
DOIs
StatePublished - Jun 2010

Keywords

  • drip emitter
  • microfluidic
  • water distribution network
  • waterborne pathogen

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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