Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission

Lonnie J. Lucas, Jennine Chesler, Jeong-Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Detection of multiple biomarkers has recently received great interest from the biosensors community. These diagnostic methods must be rapid, specific, sensitive, and cost-effective. In 2006, our group demonstrated a successful lab-on-a-chip immunoassay using microsphere light scattering, which is essentially a one-step, automated protocol, on a reusable chip. In the past, this had been difficult due to the limitations of microfluidic mixing and false-positive readings of particle immunoassays in a chip environment. In this current study, we conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD). We named this configuration "nano-on-micro" or "NOM." Upon radiation with UVlight (380 nm), a stronger light scattering signal is observed with NOMs than QDs or microspheres alone. Additionally, NOMs are easier to handle than QDs. Since QDs also provide fluorescent emission, we are able to utilize an increase in light scattering for detecting antigen-antibody reaction and a decrease in QD emission to identify which antibody (or antigen) is present. Two types of NOM combinations were used. One batch of microspheres was coated with QDs emitting at 655 nm and mouse IgG (mlgG); the other with QDs emitting at 605 nm and bovine serum albumin (BSA). A mixture of these two NOMs was used to identify either anti-mlgG or anti-BSA. NOM particles and target solutions were mixed in a microfluidic device and on-chip detection was performed using proximity optical fibers. Forward light scattering at 380 nm was collected. With the positive target, the scattering signal was increased. The LOD was 25 ng ml-1 (165 pM) with p<0.05. Fluorescent emission (655 or 605 nm) was simultaneously collected. With the positive target, the emission signal was attenuated. Therefore, we were able to detect two different antibodies simultaneously with two different detection protocols. We believe this NOM bioassay has the ability to screen for and detect multiple antibodies with minimal sample processing and handling.

Original languageEnglish (US)
Title of host publication2007 ASABE Annual International Meeting, Technical Papers
Volume15 BOOK
StatePublished - 2007
Event2007 ASABE Annual International Meeting, Technical Papers - Minneapolis, MN, United States
Duration: Jun 17 2007Jun 20 2007

Other

Other2007 ASABE Annual International Meeting, Technical Papers
CountryUnited States
CityMinneapolis, MN
Period6/17/076/20/07

Fingerprint

quantum dots
Quantum Dots
Lab-on-a-chip
light scattering
immunoassays
Microspheres
Immunoassay
Antibodies
Light scattering
Semiconductor quantum dots
Light
antibodies
Lab-On-A-Chip Devices
bovine serum albumin
Bovine Serum Albumin
Microfluidics
Limit of Detection
detection limit
Antigen-antibody reactions
antigen-antibody reactions

Keywords

  • Immunoagglutination
  • Microfluidic device
  • Multiplex assay
  • On-chip detection
  • Quantum dots
  • Static light scattering

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Engineering(all)

Cite this

Lucas, L. J., Chesler, J., & Yoon, J-Y. (2007). Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission. In 2007 ASABE Annual International Meeting, Technical Papers (Vol. 15 BOOK)

Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission. / Lucas, Lonnie J.; Chesler, Jennine; Yoon, Jeong-Yeol.

2007 ASABE Annual International Meeting, Technical Papers. Vol. 15 BOOK 2007.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lucas, LJ, Chesler, J & Yoon, J-Y 2007, Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission. in 2007 ASABE Annual International Meeting, Technical Papers. vol. 15 BOOK, 2007 ASABE Annual International Meeting, Technical Papers, Minneapolis, MN, United States, 6/17/07.
Lucas LJ, Chesler J, Yoon J-Y. Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission. In 2007 ASABE Annual International Meeting, Technical Papers. Vol. 15 BOOK. 2007
Lucas, Lonnie J. ; Chesler, Jennine ; Yoon, Jeong-Yeol. / Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission. 2007 ASABE Annual International Meeting, Technical Papers. Vol. 15 BOOK 2007.
@inproceedings{998a472152144cb7b9dbc419fa0327ce,
title = "Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission",
abstract = "Detection of multiple biomarkers has recently received great interest from the biosensors community. These diagnostic methods must be rapid, specific, sensitive, and cost-effective. In 2006, our group demonstrated a successful lab-on-a-chip immunoassay using microsphere light scattering, which is essentially a one-step, automated protocol, on a reusable chip. In the past, this had been difficult due to the limitations of microfluidic mixing and false-positive readings of particle immunoassays in a chip environment. In this current study, we conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD). We named this configuration {"}nano-on-micro{"} or {"}NOM.{"} Upon radiation with UVlight (380 nm), a stronger light scattering signal is observed with NOMs than QDs or microspheres alone. Additionally, NOMs are easier to handle than QDs. Since QDs also provide fluorescent emission, we are able to utilize an increase in light scattering for detecting antigen-antibody reaction and a decrease in QD emission to identify which antibody (or antigen) is present. Two types of NOM combinations were used. One batch of microspheres was coated with QDs emitting at 655 nm and mouse IgG (mlgG); the other with QDs emitting at 605 nm and bovine serum albumin (BSA). A mixture of these two NOMs was used to identify either anti-mlgG or anti-BSA. NOM particles and target solutions were mixed in a microfluidic device and on-chip detection was performed using proximity optical fibers. Forward light scattering at 380 nm was collected. With the positive target, the scattering signal was increased. The LOD was 25 ng ml-1 (165 pM) with p<0.05. Fluorescent emission (655 or 605 nm) was simultaneously collected. With the positive target, the emission signal was attenuated. Therefore, we were able to detect two different antibodies simultaneously with two different detection protocols. We believe this NOM bioassay has the ability to screen for and detect multiple antibodies with minimal sample processing and handling.",
keywords = "Immunoagglutination, Microfluidic device, Multiplex assay, On-chip detection, Quantum dots, Static light scattering",
author = "Lucas, {Lonnie J.} and Jennine Chesler and Jeong-Yeol Yoon",
year = "2007",
language = "English (US)",
volume = "15 BOOK",
booktitle = "2007 ASABE Annual International Meeting, Technical Papers",

}

TY - GEN

T1 - Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission

AU - Lucas, Lonnie J.

AU - Chesler, Jennine

AU - Yoon, Jeong-Yeol

PY - 2007

Y1 - 2007

N2 - Detection of multiple biomarkers has recently received great interest from the biosensors community. These diagnostic methods must be rapid, specific, sensitive, and cost-effective. In 2006, our group demonstrated a successful lab-on-a-chip immunoassay using microsphere light scattering, which is essentially a one-step, automated protocol, on a reusable chip. In the past, this had been difficult due to the limitations of microfluidic mixing and false-positive readings of particle immunoassays in a chip environment. In this current study, we conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD). We named this configuration "nano-on-micro" or "NOM." Upon radiation with UVlight (380 nm), a stronger light scattering signal is observed with NOMs than QDs or microspheres alone. Additionally, NOMs are easier to handle than QDs. Since QDs also provide fluorescent emission, we are able to utilize an increase in light scattering for detecting antigen-antibody reaction and a decrease in QD emission to identify which antibody (or antigen) is present. Two types of NOM combinations were used. One batch of microspheres was coated with QDs emitting at 655 nm and mouse IgG (mlgG); the other with QDs emitting at 605 nm and bovine serum albumin (BSA). A mixture of these two NOMs was used to identify either anti-mlgG or anti-BSA. NOM particles and target solutions were mixed in a microfluidic device and on-chip detection was performed using proximity optical fibers. Forward light scattering at 380 nm was collected. With the positive target, the scattering signal was increased. The LOD was 25 ng ml-1 (165 pM) with p<0.05. Fluorescent emission (655 or 605 nm) was simultaneously collected. With the positive target, the emission signal was attenuated. Therefore, we were able to detect two different antibodies simultaneously with two different detection protocols. We believe this NOM bioassay has the ability to screen for and detect multiple antibodies with minimal sample processing and handling.

AB - Detection of multiple biomarkers has recently received great interest from the biosensors community. These diagnostic methods must be rapid, specific, sensitive, and cost-effective. In 2006, our group demonstrated a successful lab-on-a-chip immunoassay using microsphere light scattering, which is essentially a one-step, automated protocol, on a reusable chip. In the past, this had been difficult due to the limitations of microfluidic mixing and false-positive readings of particle immunoassays in a chip environment. In this current study, we conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD). We named this configuration "nano-on-micro" or "NOM." Upon radiation with UVlight (380 nm), a stronger light scattering signal is observed with NOMs than QDs or microspheres alone. Additionally, NOMs are easier to handle than QDs. Since QDs also provide fluorescent emission, we are able to utilize an increase in light scattering for detecting antigen-antibody reaction and a decrease in QD emission to identify which antibody (or antigen) is present. Two types of NOM combinations were used. One batch of microspheres was coated with QDs emitting at 655 nm and mouse IgG (mlgG); the other with QDs emitting at 605 nm and bovine serum albumin (BSA). A mixture of these two NOMs was used to identify either anti-mlgG or anti-BSA. NOM particles and target solutions were mixed in a microfluidic device and on-chip detection was performed using proximity optical fibers. Forward light scattering at 380 nm was collected. With the positive target, the scattering signal was increased. The LOD was 25 ng ml-1 (165 pM) with p<0.05. Fluorescent emission (655 or 605 nm) was simultaneously collected. With the positive target, the emission signal was attenuated. Therefore, we were able to detect two different antibodies simultaneously with two different detection protocols. We believe this NOM bioassay has the ability to screen for and detect multiple antibodies with minimal sample processing and handling.

KW - Immunoagglutination

KW - Microfluidic device

KW - Multiplex assay

KW - On-chip detection

KW - Quantum dots

KW - Static light scattering

UR - http://www.scopus.com/inward/record.url?scp=35649006106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35649006106&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:35649006106

VL - 15 BOOK

BT - 2007 ASABE Annual International Meeting, Technical Papers

ER -