Label-free multi-photon imaging of dysplasia in Barrett’s esophagus

Soroush Mehravar, Bhaskar Banerjee, Hemant Chatrath, Babak Amirsolaimani, Krunal Patel, Charmi Patel, Robert A Norwood, Nasser N Peyghambarian, Khanh Q Kieu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Barrett’s esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of backscattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett’s.

Original languageEnglish (US)
Pages (from-to)148-157
Number of pages10
JournalBiomedical Optics Express
Volume7
Issue number1
DOIs
StatePublished - Dec 16 2015

Fingerprint

esophagus
Barrett Esophagus
Photons
Microscopy
microscopy
Biopsy
Selection Bias
photons
collagens
Cell Nucleus
fiber lasers
Lasers
Collagen
sampling
disorders
nuclei

Keywords

  • Multiphoton processes
  • Nonlinear microscopy
  • Scanning microscopy
  • Ultrafast lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Label-free multi-photon imaging of dysplasia in Barrett’s esophagus. / Mehravar, Soroush; Banerjee, Bhaskar; Chatrath, Hemant; Amirsolaimani, Babak; Patel, Krunal; Patel, Charmi; Norwood, Robert A; Peyghambarian, Nasser N; Kieu, Khanh Q.

In: Biomedical Optics Express, Vol. 7, No. 1, 16.12.2015, p. 148-157.

Research output: Contribution to journalArticle

Mehravar S, Banerjee B, Chatrath H, Amirsolaimani B, Patel K, Patel C et al. Label-free multi-photon imaging of dysplasia in Barrett’s esophagus. Biomedical Optics Express. 2015 Dec 16;7(1):148-157. https://doi.org/10.1364/BOE.7.000148
Mehravar, Soroush ; Banerjee, Bhaskar ; Chatrath, Hemant ; Amirsolaimani, Babak ; Patel, Krunal ; Patel, Charmi ; Norwood, Robert A ; Peyghambarian, Nasser N ; Kieu, Khanh Q. / Label-free multi-photon imaging of dysplasia in Barrett’s esophagus. In: Biomedical Optics Express. 2015 ; Vol. 7, No. 1. pp. 148-157.
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