Label-free multi-photon imaging of dysplasia in Barrett’s esophagus

Soroush Mehravar, Bhaskar Banerjee, Hemant Chatrath, Babak Amirsolaimani, Krunal Patel, Charmi Patel, Robert A Norwood, Nasser N Peyghambarian, Khanh Q Kieu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Barrett’s esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of backscattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett’s.

Original languageEnglish (US)
Pages (from-to)148-157
Number of pages10
JournalBiomedical Optics Express
Volume7
Issue number1
DOIs
StatePublished - Dec 16 2015

Keywords

  • Multiphoton processes
  • Nonlinear microscopy
  • Scanning microscopy
  • Ultrafast lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

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