Lack of effect of low [Ca2+], La3+, and pyrazinoate on urate transport by isolated, perfused snake renal tubules

William H Dantzler, Olga H. Brokl

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Effects of low medium calcium concentration, of lanthanum, and of pyrazinoate on urate transport by isolated, perfused snake (Thamnophis spp.) distal-proximal renal tubules were studied. Removal of calcium from perfusate with 0.18 mmol/l calcium in bathing medium had no effect on net urate secretion (J urate net ) or on net fluid absorption (J v). In the presence of calcium (1.8 mmol/l), lanthanum (2.0 mmol/l) in perfusate alone, in bathing medium alone, or in both perfusate and bathing medium had no effect on J urate net . These findings suggest that urate transport, in contrast to para-aminohippurate (PAH) transport, is not sensitive to calcium entry into the cells and support the concept that urate and PAH are transported by separate mechanisms in these renal tubules. Pyrazinoate (1.0 mmol/l) in the bathing medium had no effect on J urate net or J v. These findings do not support the idea of a primary urate secretory process uniquely sensitive to pyrazinoate among the vertebrates.

Original languageEnglish (US)
Pages (from-to)262-265
Number of pages4
JournalPflugers Archiv European Journal of Physiology
Volume401
Issue number3
DOIs
StatePublished - Jul 1984

Fingerprint

Snakes
Uric Acid
Kidney
Calcium
Lanthanum
Distal Kidney Tubule
Colubridae
Proximal Kidney Tubule
pyrazinoic acid
Secretory Pathway
Vertebrates
Fluids

Keywords

  • Calcium
  • Comparative physiology
  • Garter snakes (Thamnophis spp.)
  • Isolated renal tubules
  • Lanthanum
  • Pyrazinoate
  • Urate transport

ASJC Scopus subject areas

  • Physiology

Cite this

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abstract = "Effects of low medium calcium concentration, of lanthanum, and of pyrazinoate on urate transport by isolated, perfused snake (Thamnophis spp.) distal-proximal renal tubules were studied. Removal of calcium from perfusate with 0.18 mmol/l calcium in bathing medium had no effect on net urate secretion (J urate net ) or on net fluid absorption (J v). In the presence of calcium (1.8 mmol/l), lanthanum (2.0 mmol/l) in perfusate alone, in bathing medium alone, or in both perfusate and bathing medium had no effect on J urate net . These findings suggest that urate transport, in contrast to para-aminohippurate (PAH) transport, is not sensitive to calcium entry into the cells and support the concept that urate and PAH are transported by separate mechanisms in these renal tubules. Pyrazinoate (1.0 mmol/l) in the bathing medium had no effect on J urate net or J v. These findings do not support the idea of a primary urate secretory process uniquely sensitive to pyrazinoate among the vertebrates.",
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