Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells

Melissa Herbst-Kralovetz, Andrea L. Radtke, Margarita K. Lay, Brooke E. Hjelm, Alice N. Bolick, Shameema S. Sarker, Robert L. Atmar, David H. Kingsley, Charles J. Arntzen, Mary K. Estes, Cheryl A. Nickerson

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Noroviruses (NoVs) are a leading cause of gastroenteritis worldwide. An in vitro model for NoV replication remains elusive, making study of the virus difficult. A previous study, which used a 3-dimensional (3-D) intestinal model derived from INT-407 cells reported NoV replication and extensive cytopathic effects (CPE). Using the same 3-D model, but with highly purified Norwalk virus (NV), we attempted to replicate this study. Our results showed no evidence of NV replication by real-time PCR of viral RNA or by immunocytochemical detection of viral structural and nonstructural proteins. Immunocytochemical analysis of the 3-D cultures also showed no detectable presence of histo-blood group antigens that participate in NV binding and host tropism. To determine the potential cause of CPE observed in the previous study, we exposed 3-D cultures to lipopolysaccharide concentrations consistent with contaminated stool samples and observed morphologic features similar to CPE. We conclude that the 3-D INT-407 model does not support NV replication.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalEmerging Infectious Diseases
Volume19
Issue number3
DOIs
StatePublished - Mar 2013

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Norwalk virus
Norovirus
Blood Group Antigens
Epithelial Cells
Virus Replication
Viral Nonstructural Proteins
Viral Tropism
Viral Structural Proteins
Virus Attachment
Viral RNA
Gastroenteritis
Lipopolysaccharides
Real-Time Polymerase Chain Reaction
Viruses

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells. / Herbst-Kralovetz, Melissa; Radtke, Andrea L.; Lay, Margarita K.; Hjelm, Brooke E.; Bolick, Alice N.; Sarker, Shameema S.; Atmar, Robert L.; Kingsley, David H.; Arntzen, Charles J.; Estes, Mary K.; Nickerson, Cheryl A.

In: Emerging Infectious Diseases, Vol. 19, No. 3, 03.2013, p. 431-438.

Research output: Contribution to journalArticle

Herbst-Kralovetz, M, Radtke, AL, Lay, MK, Hjelm, BE, Bolick, AN, Sarker, SS, Atmar, RL, Kingsley, DH, Arntzen, CJ, Estes, MK & Nickerson, CA 2013, 'Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells', Emerging Infectious Diseases, vol. 19, no. 3, pp. 431-438. https://doi.org/10.3201/eid1903.121029
Herbst-Kralovetz, Melissa ; Radtke, Andrea L. ; Lay, Margarita K. ; Hjelm, Brooke E. ; Bolick, Alice N. ; Sarker, Shameema S. ; Atmar, Robert L. ; Kingsley, David H. ; Arntzen, Charles J. ; Estes, Mary K. ; Nickerson, Cheryl A. / Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells. In: Emerging Infectious Diseases. 2013 ; Vol. 19, No. 3. pp. 431-438.
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