Laminin Alpha-1, Alpha-3, and Alpha-5 chain expression in human prepubetal benign prostate glands and adult benign and malignant prostate glands

Preetinder K. Brar, Bruce L. Dalkin, Chris Weyer, Karim Sallam, Ismo Virtanen, Raymond B Nagle

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

BACKGROUND. Laminins (Lns) are a family of extracellular matrix glycoproteins located in the basement membrane (BM) of epithelial cells. They exist as heterotrimers composed of an α, β, and γ chain. Presently, five α (α1-5), three β (β1-3), and three γ (γ1-3) chains have been identified with different combinations of these chains resulting in 14 laminin heterotrimers thus far identified (1, 3-5). METHODS. In this study, using immunohistochemistry with chain-specific antibodies, we characterized the expression of the α1 (Lns-1/3), α3 (Lns 5,6,7), and α5 (Lns 10/11) chains in fetal, newborn, infant, prepubertal, and adult benign and malignant prostate glands. RESULTS. In general, α1 expression was higher in normal fetal prostate glands and declined by full-term birth, whereas the α3 and α5 chains remained highly expressed in the adult normal glands. In carcinoma α1 (Lns 1/3) and α5 (Lns 5,6,7) are lost, whereas α5 (Lns 10/11) persists. CONCLUSIONS. Alpha 1 (Lns 1/3) is prominent in BM, but is replaced by a laminin matrix rich in α3 (Lns 5,6,7) and α5 (Lns 10/11) in benign adult prostate glands. In carcinoma, both α1 (Lns-1/3) and α3 (Lns 5,6,7) are not expressed with persistence of a BM rich in α5 (Lns 10/11).

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalProstate
Volume55
Issue number1
DOIs
StatePublished - Apr 1 2003

Fingerprint

Prostate
Laminin
Basement Membrane
Term Birth
Carcinoma
Extracellular Matrix
Glycoproteins
Epithelial Cells
Immunohistochemistry
laminin 1
kalinin
Newborn Infant
laminin 10
laminin 6
Antibodies

Keywords

  • Laminin chains
  • Prepubertal prostate

ASJC Scopus subject areas

  • Urology

Cite this

Laminin Alpha-1, Alpha-3, and Alpha-5 chain expression in human prepubetal benign prostate glands and adult benign and malignant prostate glands. / Brar, Preetinder K.; Dalkin, Bruce L.; Weyer, Chris; Sallam, Karim; Virtanen, Ismo; Nagle, Raymond B.

In: Prostate, Vol. 55, No. 1, 01.04.2003, p. 65-70.

Research output: Contribution to journalArticle

Brar, Preetinder K. ; Dalkin, Bruce L. ; Weyer, Chris ; Sallam, Karim ; Virtanen, Ismo ; Nagle, Raymond B. / Laminin Alpha-1, Alpha-3, and Alpha-5 chain expression in human prepubetal benign prostate glands and adult benign and malignant prostate glands. In: Prostate. 2003 ; Vol. 55, No. 1. pp. 65-70.
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abstract = "BACKGROUND. Laminins (Lns) are a family of extracellular matrix glycoproteins located in the basement membrane (BM) of epithelial cells. They exist as heterotrimers composed of an α, β, and γ chain. Presently, five α (α1-5), three β (β1-3), and three γ (γ1-3) chains have been identified with different combinations of these chains resulting in 14 laminin heterotrimers thus far identified (1, 3-5). METHODS. In this study, using immunohistochemistry with chain-specific antibodies, we characterized the expression of the α1 (Lns-1/3), α3 (Lns 5,6,7), and α5 (Lns 10/11) chains in fetal, newborn, infant, prepubertal, and adult benign and malignant prostate glands. RESULTS. In general, α1 expression was higher in normal fetal prostate glands and declined by full-term birth, whereas the α3 and α5 chains remained highly expressed in the adult normal glands. In carcinoma α1 (Lns 1/3) and α5 (Lns 5,6,7) are lost, whereas α5 (Lns 10/11) persists. CONCLUSIONS. Alpha 1 (Lns 1/3) is prominent in BM, but is replaced by a laminin matrix rich in α3 (Lns 5,6,7) and α5 (Lns 10/11) in benign adult prostate glands. In carcinoma, both α1 (Lns-1/3) and α3 (Lns 5,6,7) are not expressed with persistence of a BM rich in α5 (Lns 10/11).",
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T1 - Laminin Alpha-1, Alpha-3, and Alpha-5 chain expression in human prepubetal benign prostate glands and adult benign and malignant prostate glands

AU - Brar, Preetinder K.

AU - Dalkin, Bruce L.

AU - Weyer, Chris

AU - Sallam, Karim

AU - Virtanen, Ismo

AU - Nagle, Raymond B

PY - 2003/4/1

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N2 - BACKGROUND. Laminins (Lns) are a family of extracellular matrix glycoproteins located in the basement membrane (BM) of epithelial cells. They exist as heterotrimers composed of an α, β, and γ chain. Presently, five α (α1-5), three β (β1-3), and three γ (γ1-3) chains have been identified with different combinations of these chains resulting in 14 laminin heterotrimers thus far identified (1, 3-5). METHODS. In this study, using immunohistochemistry with chain-specific antibodies, we characterized the expression of the α1 (Lns-1/3), α3 (Lns 5,6,7), and α5 (Lns 10/11) chains in fetal, newborn, infant, prepubertal, and adult benign and malignant prostate glands. RESULTS. In general, α1 expression was higher in normal fetal prostate glands and declined by full-term birth, whereas the α3 and α5 chains remained highly expressed in the adult normal glands. In carcinoma α1 (Lns 1/3) and α5 (Lns 5,6,7) are lost, whereas α5 (Lns 10/11) persists. CONCLUSIONS. Alpha 1 (Lns 1/3) is prominent in BM, but is replaced by a laminin matrix rich in α3 (Lns 5,6,7) and α5 (Lns 10/11) in benign adult prostate glands. In carcinoma, both α1 (Lns-1/3) and α3 (Lns 5,6,7) are not expressed with persistence of a BM rich in α5 (Lns 10/11).

AB - BACKGROUND. Laminins (Lns) are a family of extracellular matrix glycoproteins located in the basement membrane (BM) of epithelial cells. They exist as heterotrimers composed of an α, β, and γ chain. Presently, five α (α1-5), three β (β1-3), and three γ (γ1-3) chains have been identified with different combinations of these chains resulting in 14 laminin heterotrimers thus far identified (1, 3-5). METHODS. In this study, using immunohistochemistry with chain-specific antibodies, we characterized the expression of the α1 (Lns-1/3), α3 (Lns 5,6,7), and α5 (Lns 10/11) chains in fetal, newborn, infant, prepubertal, and adult benign and malignant prostate glands. RESULTS. In general, α1 expression was higher in normal fetal prostate glands and declined by full-term birth, whereas the α3 and α5 chains remained highly expressed in the adult normal glands. In carcinoma α1 (Lns 1/3) and α5 (Lns 5,6,7) are lost, whereas α5 (Lns 10/11) persists. CONCLUSIONS. Alpha 1 (Lns 1/3) is prominent in BM, but is replaced by a laminin matrix rich in α3 (Lns 5,6,7) and α5 (Lns 10/11) in benign adult prostate glands. In carcinoma, both α1 (Lns-1/3) and α3 (Lns 5,6,7) are not expressed with persistence of a BM rich in α5 (Lns 10/11).

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EP - 70

JO - Prostate

JF - Prostate

SN - 0270-4137

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