Laminin Alpha-1, Alpha-3, and Alpha-5 chain expression in human prepubetal benign prostate glands and adult benign and malignant prostate glands

Preetinder K. Brar, Bruce L. Dalkin, Chris Weyer, Karim Sallam, Ismo Virtanen, Raymond B. Nagle

Research output: Contribution to journalArticle

24 Scopus citations


BACKGROUND. Laminins (Lns) are a family of extracellular matrix glycoproteins located in the basement membrane (BM) of epithelial cells. They exist as heterotrimers composed of an α, β, and γ chain. Presently, five α (α1-5), three β (β1-3), and three γ (γ1-3) chains have been identified with different combinations of these chains resulting in 14 laminin heterotrimers thus far identified (1, 3-5). METHODS. In this study, using immunohistochemistry with chain-specific antibodies, we characterized the expression of the α1 (Lns-1/3), α3 (Lns 5,6,7), and α5 (Lns 10/11) chains in fetal, newborn, infant, prepubertal, and adult benign and malignant prostate glands. RESULTS. In general, α1 expression was higher in normal fetal prostate glands and declined by full-term birth, whereas the α3 and α5 chains remained highly expressed in the adult normal glands. In carcinoma α1 (Lns 1/3) and α5 (Lns 5,6,7) are lost, whereas α5 (Lns 10/11) persists. CONCLUSIONS. Alpha 1 (Lns 1/3) is prominent in BM, but is replaced by a laminin matrix rich in α3 (Lns 5,6,7) and α5 (Lns 10/11) in benign adult prostate glands. In carcinoma, both α1 (Lns-1/3) and α3 (Lns 5,6,7) are not expressed with persistence of a BM rich in α5 (Lns 10/11).

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
Issue number1
StatePublished - Apr 1 2003



  • Laminin chains
  • Prepubertal prostate

ASJC Scopus subject areas

  • Oncology
  • Urology

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