Lanosterol and Cholesterol-Induced Variations in Bilayer Elasticity Probed by 2H NMR Relaxation

Gary V. Martinez, Emily M. Dykstra, Silvia Lope-Piedrafita, Michael F Brown

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The influences of lanosterol on lipid bilayers have been compared to those of cholesterol by combining deuterium ( 2H) NMR spin relaxation studies with segmental order parameter measurements. For bilayers of 1,2-diperdeuteriomyristoyl-sn-glycero-3-phosphocholine (DMPC-d 54), the results are consistent with a square-law dependence of the 2H Zeeman relaxation rates (R 1Z) on the corresponding order parameters (S CD). This behavior is indicative of relatively slow order fluctuations, for example, due to quasi-elastic bilayer disturbances. Significant differences are found in the influences of lanosterol versus cholesterol on the microscopic NMR observables; although lanosterol produces smaller order parameters than cholesterol, it leads to larger relaxation rates. By correlating the NMR relaxation behavior with the order parameters, the results are explained by a progressive reduction of the bilayer elasticity, which parallels the biosynthetic pathway from lanosterol to cholesterol.

Original languageEnglish (US)
Pages (from-to)1043-1046
Number of pages4
JournalLangmuir
Volume20
Issue number4
DOIs
StatePublished - Feb 17 2004

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Lanosterol
Cholesterol
cholesterol
Elasticity
elastic properties
Nuclear magnetic resonance
nuclear magnetic resonance
Dimyristoylphosphatidylcholine
Lipid bilayers
Phosphorylcholine
Deuterium
lipids
deuterium
disturbances

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Lanosterol and Cholesterol-Induced Variations in Bilayer Elasticity Probed by 2H NMR Relaxation. / Martinez, Gary V.; Dykstra, Emily M.; Lope-Piedrafita, Silvia; Brown, Michael F.

In: Langmuir, Vol. 20, No. 4, 17.02.2004, p. 1043-1046.

Research output: Contribution to journalArticle

Martinez, Gary V. ; Dykstra, Emily M. ; Lope-Piedrafita, Silvia ; Brown, Michael F. / Lanosterol and Cholesterol-Induced Variations in Bilayer Elasticity Probed by 2H NMR Relaxation. In: Langmuir. 2004 ; Vol. 20, No. 4. pp. 1043-1046.
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