Laparoscopic bowel injury in an animal model: Monocyte migration and apoptosis

A. El-Hakim, J. P.A. Aldana, K. Reddy, P. Singhal, B. R. Lee

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background: Unrecognized laparoscopic bowel injury has a delayed and covert presentation. Differences in monocyte migration and apoptosis between laparoscopic and open bowel injury were determined. Methods: For this study, 24 rabbits were divided into laparoscopic (n = 9) and open surgical (n = 9) bowel injury groups and a control group (n = 6) without bowel injury. Bowel injury was created using monopolar electrocautery. The animals were killed 1 day, 1 week, and 2 weeks after surgery. Monocyte migration assay was performed across a modified Boyden chamber. Apoptosis was assessed by DNA fluorescent stain H-33342. Results: In laparoscopy, monocyte apoptosis was decreased (p < 0.001), and migration was increased (p < 0.05), as compared with the open group. Apoptosis increased over time in both study groups, and was higher than in the control group (p < 0.001). Migration was decreased in both study groups as compared with the control group (p < 0.05) Conclusions: These results suggest decreased immune system priming with laparoscopic bowel injury, which may contribute to the masking of relevant signs and symptoms of peritonitis.

Original languageEnglish (US)
Pages (from-to)484-487
Number of pages4
JournalSurgical Endoscopy and Other Interventional Techniques
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2005
Externally publishedYes

Keywords

  • Apoptosis
  • Cautery
  • Intestinal perforation
  • Laparoscopy
  • Macrophage migration inhibitory factors
  • Monocytes

ASJC Scopus subject areas

  • Surgery

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