Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo

Dongkyun Kang, Simon C. Schlachter, Robert W. Carruth, Minkyu Kim, Tao Wu, Nima Tabatabaei, Amna R. Soomro, Catriona N. Grant, Mireille Rosenberg, Norman S. Nishioka, Guillermo J. Tearney

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and Objective: Diagnosis of esophageal diseases is often hampered by sampling errors that are inherent in endoscopic biopsy, the standard of care. Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance confocal endomicroscopy technology that has the potential to visualize cellular features from large regions of the esophagus, greatly decreasing the likelihood of sampling error. In this paper, we report results from a pilot clinical study imaging the human esophagus in vivo with a prototype SECM endoscopic probe. Materials and Methods: In this pilot clinical study, six patients undergoing esophagogastroduodenoscopy (EGD) for surveillance of Barrett's esophagus (BE) were imaged with the SECM endoscopic probe. The device had a diameter of 7 mm, a length of 2 m, and a rapid-exchange guide wire provision for esophageal placement. During EGD, the distal portion of the esophagus of each patient was sprayed with 2.5% acetic acid to enhance nuclear contrast. The SECM endoscopic probe was then introduced over the guide wire to the distal esophagus and large-area confocal images were obtained by helically scanning the optics within the SECM probe. Results: Large area confocal images of the distal esophagus (image length = 4.3–10 cm; image width = 2.2 cm) were rapidly acquired at a rate of ∼9 mm2/second, resulting in short procedural times (1.8–4 minutes). SECM enabled the visualization of clinically relevant architectural and cellular features of the proximal stomach and normal and diseased esophagus, including squamous cell nuclei, BE glands, and goblet cells. Conclusions: This study demonstrates that comprehensive spectrally encoded confocal endomicroscopy is feasible and can be used to visualize architectural and cellular microscopic features from large segments of the distal esophagus at the gastroesophageal junction. By providing microscopic images that are less subject to sampling error, this technology may find utility in guiding biopsy and planning and assessing endoscopic therapy. Lasers Surg. Med. 49:233–239, 2017.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalLasers in Surgery and Medicine
Volume49
Issue number3
DOIs
StatePublished - Mar 1 2017
Externally publishedYes

Fingerprint

Esophagus
Confocal Microscopy
Selection Bias
Digestive System Endoscopy
Barrett Esophagus
Esophageal Diseases
Technology
Biopsy
Stomach Diseases
Esophagogastric Junction
Goblet Cells
Standard of Care
Cell Nucleus
Acetic Acid
Lasers
Epithelial Cells
Equipment and Supplies

Keywords

  • Barrett's esophagus
  • confocal endomicroscopy
  • esophageal imaging
  • reflectance confocal microcopy
  • spectrally encoded confocal microscopy

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Kang, D., Schlachter, S. C., Carruth, R. W., Kim, M., Wu, T., Tabatabaei, N., ... Tearney, G. J. (2017). Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo. Lasers in Surgery and Medicine, 49(3), 233-239. https://doi.org/10.1002/lsm.22585

Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo. / Kang, Dongkyun; Schlachter, Simon C.; Carruth, Robert W.; Kim, Minkyu; Wu, Tao; Tabatabaei, Nima; Soomro, Amna R.; Grant, Catriona N.; Rosenberg, Mireille; Nishioka, Norman S.; Tearney, Guillermo J.

In: Lasers in Surgery and Medicine, Vol. 49, No. 3, 01.03.2017, p. 233-239.

Research output: Contribution to journalArticle

Kang, D, Schlachter, SC, Carruth, RW, Kim, M, Wu, T, Tabatabaei, N, Soomro, AR, Grant, CN, Rosenberg, M, Nishioka, NS & Tearney, GJ 2017, 'Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo', Lasers in Surgery and Medicine, vol. 49, no. 3, pp. 233-239. https://doi.org/10.1002/lsm.22585
Kang, Dongkyun ; Schlachter, Simon C. ; Carruth, Robert W. ; Kim, Minkyu ; Wu, Tao ; Tabatabaei, Nima ; Soomro, Amna R. ; Grant, Catriona N. ; Rosenberg, Mireille ; Nishioka, Norman S. ; Tearney, Guillermo J. / Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo. In: Lasers in Surgery and Medicine. 2017 ; Vol. 49, No. 3. pp. 233-239.
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AU - Wu, Tao

AU - Tabatabaei, Nima

AU - Soomro, Amna R.

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