Large-image-format computed tomography imaging spectrometer for fluorescence microscopy

Bridget K. Ford, Michael R. Descour, Ronald M. Lynch

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Multispectral imaging has significantly enhanced the analysis of fixed specimens in pathology and cytogenetics. However, application of this technology to in vivo studies has been limited. This is due in part to the increased temporal resolution required to analyze changes in cellular function. Here we present a non-scanning instrument that simultaneously acquires full spectral information (460 nm to 740 nm) from every pixel within its 2-D field of view (200 μm × 200 μm) during a single integration time (typically, 2 seconds). The current spatial and spectral sampling intervals of the spectrometer are 0.985 μm and 5 nm, respectively. These properties allow for the analysis of physiological responses within living biological specimens.

Original languageEnglish (US)
Pages (from-to)444-453
Number of pages10
JournalOptics Express
Volume9
Issue number9
DOIs
StatePublished - Oct 2001

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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