Large-scale maps of variable infection efficiencies in aquatic Bacteroidetes phage-host model systems

Karin Holmfeldt, Natalie Solonenko, Cristina Howard-Varona, Mario Moreno, Rex R. Malmstrom, Matthew J. Blow, Matthew Sullivan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Microbes drive ecosystem functioning and their viruses modulate these impacts through mortality, gene transfer and metabolic reprogramming. Despite the importance of virus-host interactions and likely variable infection efficiencies of individual phages across hosts, such variability is seldom quantified. Here, we quantify infection efficiencies of 38 phages against 19 host strains in aquatic Cellulophaga (Bacteroidetes) phage-host model systems. Binary data revealed that some phages infected only one strain while others infected 17, whereas quantitative data revealed that efficiency of infection could vary 10 orders of magnitude, even among phages within one population. This provides a baseline for understanding and modeling intrapopulation host range variation. Genera specific host ranges were also informative. For example, the Cellulophaga Microviridae, showed a markedly broader intra-species host range than previously observed in Escherichia coli systems. Further, one phage genus, Cba41, was examined to investigate nonheritable changes in plating efficiency and burst size that depended on which host strain it most recently infected. While consistent with host modification of phage DNA, no differences in nucleotide sequence or DNA modifications were detected, leaving the observation repeatable, but the mechanism unresolved. Overall, this study highlights the importance of quantitatively considering replication variations in studies of phage-host interactions.

Original languageEnglish (US)
JournalEnvironmental Microbiology
DOIs
StateAccepted/In press - 2016

Fingerprint

Bacteroidetes
bacteriophages
Bacteriophages
Infection
host range
infection
Cellulophaga
Host Specificity
host strains
Microviridae
virus
DNA
Viruses
gene transfer
viruses
Ecosystem
mortality
Observation
Escherichia coli
microorganisms

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Large-scale maps of variable infection efficiencies in aquatic Bacteroidetes phage-host model systems. / Holmfeldt, Karin; Solonenko, Natalie; Howard-Varona, Cristina; Moreno, Mario; Malmstrom, Rex R.; Blow, Matthew J.; Sullivan, Matthew.

In: Environmental Microbiology, 2016.

Research output: Contribution to journalArticle

Holmfeldt, Karin ; Solonenko, Natalie ; Howard-Varona, Cristina ; Moreno, Mario ; Malmstrom, Rex R. ; Blow, Matthew J. ; Sullivan, Matthew. / Large-scale maps of variable infection efficiencies in aquatic Bacteroidetes phage-host model systems. In: Environmental Microbiology. 2016.
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