Large-scale photoautotrophic micropropagation in a scaled-up vessel

S. M A Zobayed, F. Afreen, Chieri Kubota, T. Kozai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A scaled-up culture vessel for the large-scale micropropagation has been designed and the method for the large-scale micro propagation of Eucalyptus under photoauto trophic condition (sugar-free medium) using this scaled-up culture vessel with forced ventilation (SV-treatment) is described. Growth, multiplication, epicuticular wax content, stomatal function of plantlets and transpiration rate and ex vitro survival of the plants grown under SV-treatment were studied and compared with those of the plantlets grown in a Magenta-type vessel with natural ventilation and under photomixotrophic condition (30 g l-1 sucrose in the medium). Results revealed that fresh and dry mass in plantlets grown under SV-treatment were significantly higher than those of plantlets from Magenta type vessel. Multiplication was enhanced and normal stomatal closing and opening were observed in the leaves of plantlets grown in scaled-up vessel. Epicuticular leaf-wax content was significantly higher compared with that of the leaves grown in Magenta type vessel. The anatomical study showed well-organized palisade and spongy mesophyll layers for leaves grown in SV-treatment. Importantly after transplanting ex vitro, plantlets survived well without any specialized ex vitro acclimatization treatment. This was because they were better able to control transpiration and thus lost less water and showed no signs of wilting. In contrast, plantlets cultured in Magenta type vessel had open stomata which were not functional, showed a higher transpiration rate and uncontrolled and rapid water loss immediately after transferring ex vitro. Minimization of large-scale plantlet production cost by using scaled-up vessel is also discussed in this article.

Original languageEnglish (US)
Pages (from-to)345-354
Number of pages10
JournalProgress in Biotechnology
Volume18
Issue numberC
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Waxes
Ventilation
Eucalyptus
Water
Acclimatization
Sucrose
Costs and Cost Analysis
Growth

Keywords

  • Ex vitro
  • forced ventilation
  • photoautotrophic
  • stomata
  • wax content

ASJC Scopus subject areas

  • Biotechnology

Cite this

Large-scale photoautotrophic micropropagation in a scaled-up vessel. / Zobayed, S. M A; Afreen, F.; Kubota, Chieri; Kozai, T.

In: Progress in Biotechnology, Vol. 18, No. C, 2001, p. 345-354.

Research output: Contribution to journalArticle

Zobayed, S. M A ; Afreen, F. ; Kubota, Chieri ; Kozai, T. / Large-scale photoautotrophic micropropagation in a scaled-up vessel. In: Progress in Biotechnology. 2001 ; Vol. 18, No. C. pp. 345-354.
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