Lasp-2 expression, localization, and ligand interactions: A new Z-disc scaffolding protein

Anke Zieseniss, Asako G. Terasaki, Carol Gregorio

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The nebulin family of actin-binding proteins plays an important role in actin filament dynamics in a variety of cells including striated muscle. We report here the identification of a new striated muscle Z-disc associated protein: lasp-2 (LIM and SH3 domain protein-2). Lasp-2 is the most recently identified member of the nebulin family. To evaluate the role of lasp-2 in striated muscle, lasp-2 gene expression and localization were studied in chick and mouse tissue, as well as in primary cultures of chick cardiac and skeletal myocytes. Lasp-2 mRNA was detected as early as chick embryonic stage 25 and lasp-2 protein was associated with developing premyofibril structures, Z-discs of mature myofibrils, focal adhesions, and intercalated discs of cultured cardiomyocytes. Expression of GFP-tagged lasp-2 deletion constructs showed that the C-terminal region of lasp-2 is important for its localization in striated muscle cells. Lasp-2 organizes actin filaments into bundles and interacts directly with the Z-disc protein alpha-actinin. These results are consistent with a function of lasp-2 as a scaffolding and actin filament organizing protein within striated muscle Z-discs.

Original languageEnglish (US)
Pages (from-to)59-72
Number of pages14
JournalCell Motility and the Cytoskeleton
Volume65
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

LIM Domain Proteins
src Homology Domains
Striated Muscle
Ligands
Actin Cytoskeleton
Proteins
Cardiac Myocytes
Actinin
Microfilament Proteins
Focal Adhesions
Myofibrils
Skeletal Muscle Fibers
Muscle Cells
Gene Expression
Messenger RNA

Keywords

  • Alpha-actinin
  • Nebulin
  • Sarcomere
  • Thin filaments

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lasp-2 expression, localization, and ligand interactions : A new Z-disc scaffolding protein. / Zieseniss, Anke; Terasaki, Asako G.; Gregorio, Carol.

In: Cell Motility and the Cytoskeleton, Vol. 65, No. 1, 01.2008, p. 59-72.

Research output: Contribution to journalArticle

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