Lasp-2 expression, localization, and ligand interactions: A new Z-disc scaffolding protein

Anke Zieseniss, Asako G. Terasaki, Carol Gregorio

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The nebulin family of actin-binding proteins plays an important role in actin filament dynamics in a variety of cells including striated muscle. We report here the identification of a new striated muscle Z-disc associated protein: lasp-2 (LIM and SH3 domain protein-2). Lasp-2 is the most recently identified member of the nebulin family. To evaluate the role of lasp-2 in striated muscle, lasp-2 gene expression and localization were studied in chick and mouse tissue, as well as in primary cultures of chick cardiac and skeletal myocytes. Lasp-2 mRNA was detected as early as chick embryonic stage 25 and lasp-2 protein was associated with developing premyofibril structures, Z-discs of mature myofibrils, focal adhesions, and intercalated discs of cultured cardiomyocytes. Expression of GFP-tagged lasp-2 deletion constructs showed that the C-terminal region of lasp-2 is important for its localization in striated muscle cells. Lasp-2 organizes actin filaments into bundles and interacts directly with the Z-disc protein alpha-actinin. These results are consistent with a function of lasp-2 as a scaffolding and actin filament organizing protein within striated muscle Z-discs.

Original languageEnglish (US)
Pages (from-to)59-72
Number of pages14
JournalCell Motility and the Cytoskeleton
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 2008

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Keywords

  • Alpha-actinin
  • Nebulin
  • Sarcomere
  • Thin filaments

ASJC Scopus subject areas

  • Cell Biology

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