Lateralized kappa opioid receptor signaling from the amygdala central nucleus promotes stress-induced functional pain

Kelsey M. Nation, Milena De Felice, Pablo I. Hernandez, David W. Dodick, Volker Neugebauer, Edita Navratilova, Frank Porreca

Research output: Contribution to journalArticle

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Abstract

The response of diffuse noxious inhibitory controls (DNIC) is often decreased, or lost, in stress-related functional pain syndromes. Because the dynorphin/kappa opioid receptor (KOR) pathway is activated by stress, we determined its role in DNIC using a model of stress-induced functional pain. Male, Sprague-Dawley rats were primed for 7 days with systemic morphine resulting in opioid-induced hyperalgesia. Fourteen days after priming, when hyperalgesia was resolved, rats were exposed to environmental stress and DNIC was evaluated by measuring hind paw response threshold to noxious pressure (test stimulus) after capsaicin injection in the forepaw (conditioning stimulus). Morphine priming without stress did not alter DNIC. However, stress produced a loss of DNIC in morphine-primed rats in both hind paws that was abolished by systemic administration of the KOR antagonist, nor-binaltorphimine (nor-BNI). Microinjection of nor-BNI into the right, but not left, central nucleus of the amygdala (CeA) prevented the loss of DNIC in morphine-primed rats. Diffuse noxious inhibitory controls were not modulated by bilateral nor-BNI in the rostral ventromedial medulla. Stress increased dynorphin content in both the left and right CeA of primed rats, reaching significance only in the right CeA; no change was observed in the rostral ventromedial medulla or hypothalamus. Although morphine priming alone is not sufficient to influence DNIC, it establishes a state of latent sensitization that amplifies the consequences of stress. After priming, stress-induced dynorphin/KOR signaling from the right CeA inhibits DNIC in both hind paws, likely reflecting enhanced descending facilitation that masks descending inhibition. Kappa opioid receptor antagonists may provide a new therapeutic strategy for stress-related functional pain disorders.

Original languageEnglish (US)
Pages (from-to)919-928
Number of pages10
JournalPain
Volume159
Issue number5
DOIs
StatePublished - May 1 2018

Fingerprint

Diffuse Noxious Inhibitory Control
kappa Opioid Receptor
Pain
Morphine
Dynorphins
Narcotic Antagonists
Hyperalgesia
Central Amygdaloid Nucleus
Somatoform Disorders
Capsaicin
Microinjections
Masks
Opioid Analgesics
Hypothalamus
Sprague Dawley Rats

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Nation, K. M., De Felice, M., Hernandez, P. I., Dodick, D. W., Neugebauer, V., Navratilova, E., & Porreca, F. (2018). Lateralized kappa opioid receptor signaling from the amygdala central nucleus promotes stress-induced functional pain. Pain, 159(5), 919-928. https://doi.org/10.1097/j.pain.0000000000001167

Lateralized kappa opioid receptor signaling from the amygdala central nucleus promotes stress-induced functional pain. / Nation, Kelsey M.; De Felice, Milena; Hernandez, Pablo I.; Dodick, David W.; Neugebauer, Volker; Navratilova, Edita; Porreca, Frank.

In: Pain, Vol. 159, No. 5, 01.05.2018, p. 919-928.

Research output: Contribution to journalArticle

Nation, KM, De Felice, M, Hernandez, PI, Dodick, DW, Neugebauer, V, Navratilova, E & Porreca, F 2018, 'Lateralized kappa opioid receptor signaling from the amygdala central nucleus promotes stress-induced functional pain', Pain, vol. 159, no. 5, pp. 919-928. https://doi.org/10.1097/j.pain.0000000000001167
Nation KM, De Felice M, Hernandez PI, Dodick DW, Neugebauer V, Navratilova E et al. Lateralized kappa opioid receptor signaling from the amygdala central nucleus promotes stress-induced functional pain. Pain. 2018 May 1;159(5):919-928. https://doi.org/10.1097/j.pain.0000000000001167
Nation, Kelsey M. ; De Felice, Milena ; Hernandez, Pablo I. ; Dodick, David W. ; Neugebauer, Volker ; Navratilova, Edita ; Porreca, Frank. / Lateralized kappa opioid receptor signaling from the amygdala central nucleus promotes stress-induced functional pain. In: Pain. 2018 ; Vol. 159, No. 5. pp. 919-928.
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