Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device

Brian C. Heinze, Jae Young Song, Jin Hee Han, Jeong-Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We have investigated the utilization of particle agglutination assays using forward light scattering measurements in a microfluidic device towards detecting viral particles. The model viral target was bovine viral diarrhea virus (BVDV). Highly carboxylated polystyrene microspheres (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution was in turn used to detect live modified BVDV. This assay was first performed in a two well slide for proof of concept and then in a simple y-channel microfluidic device with optical fibers arranged in a close proximity setup. Particle immunoagglutination was detected through static light scattering measurements taken at 45° to incident light. In the microfluidic device, modified live BVDV was detected with a detection limit of 0.5 TCID50 mL-1.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume6886
DOIs
Publication statusPublished - 2008
EventMicrofluidics, BioMEMS, and Medical Microsystems VI - San Jose, CA, United States
Duration: Jan 21 2008Jan 22 2008

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems VI
CountryUnited States
CitySan Jose, CA
Period1/21/081/22/08

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Heinze, B. C., Song, J. Y., Han, J. H., & Yoon, J-Y. (2008). Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6886). [688605] https://doi.org/10.1117/12.761341