Latrophilin-2 is a novel component of the epithelial-mesenchymal transition within the atrioventricular canal of the embryonic chicken heart

Sally E. Doyle, Matthew J. Scholz, Kevin A. Greer, Antony D. Hubbard, Diana K. Darnell, Parker B Antin, Scott E Klewer, Raymond B Runyan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Endothelial cells in the atrioventricular canal of the heart undergo an epithelial-mesenchymal transition (EMT) to form heart valves. We surveyed an on-line database (http://www.geisha.arizona.edu/) for clones expressed during gastrulation to identify novel EMT components. One gene, latrophilin-2, was identified as expressed in the heart and appeared to be functional in EMT. This molecule was chosen for further examination. In situ localization showed it to be expressed in both the myocardium and endothelium. Several antisense DNA probes and an siRNA for latrophilin-2 produced a loss of EMT in collagen gel cultures. Latrophilin-2 is a putative G-protein-coupled receptor and we previously identified a pertussis toxin-sensitive G-protein signal transduction pathway. Microarray experiments were performed to examine whether these molecules were related. After treatment with antisense DNA against latrophilin-2, expression of 1,385 genes and ESTs was altered. This represented approximately 12.5% of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9%) elements of the array. There appears to be little overlap between the two signal transduction pathways. Latrophilin-2 is thus a novel component of EMT and provides a new avenue for investigation of this cellular process.

Original languageEnglish (US)
Pages (from-to)3213-3221
Number of pages9
JournalDevelopmental Dynamics
Volume235
Issue number12
DOIs
StatePublished - Dec 2006

Fingerprint

Epithelial-Mesenchymal Transition
Chickens
Antisense DNA
Pertussis Toxin
Signal Transduction
Gastrulation
Expressed Sequence Tags
Heart Valves
DNA Probes
G-Protein-Coupled Receptors
GTP-Binding Proteins
Small Interfering RNA
Genes
Endothelium
Myocardium
Collagen
Endothelial Cells
Clone Cells
Gels
alpha-latrotoxin receptor

Keywords

  • Cardiac cushions
  • Cardiac development
  • EMT
  • Microarray
  • Pertussis toxin
  • TGF-beta
  • Valvulosepatal development

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Latrophilin-2 is a novel component of the epithelial-mesenchymal transition within the atrioventricular canal of the embryonic chicken heart. / Doyle, Sally E.; Scholz, Matthew J.; Greer, Kevin A.; Hubbard, Antony D.; Darnell, Diana K.; Antin, Parker B; Klewer, Scott E; Runyan, Raymond B.

In: Developmental Dynamics, Vol. 235, No. 12, 12.2006, p. 3213-3221.

Research output: Contribution to journalArticle

Doyle, Sally E. ; Scholz, Matthew J. ; Greer, Kevin A. ; Hubbard, Antony D. ; Darnell, Diana K. ; Antin, Parker B ; Klewer, Scott E ; Runyan, Raymond B. / Latrophilin-2 is a novel component of the epithelial-mesenchymal transition within the atrioventricular canal of the embryonic chicken heart. In: Developmental Dynamics. 2006 ; Vol. 235, No. 12. pp. 3213-3221.
@article{a75b7606e8b844cf94bcecc9df97a29f,
title = "Latrophilin-2 is a novel component of the epithelial-mesenchymal transition within the atrioventricular canal of the embryonic chicken heart",
abstract = "Endothelial cells in the atrioventricular canal of the heart undergo an epithelial-mesenchymal transition (EMT) to form heart valves. We surveyed an on-line database (http://www.geisha.arizona.edu/) for clones expressed during gastrulation to identify novel EMT components. One gene, latrophilin-2, was identified as expressed in the heart and appeared to be functional in EMT. This molecule was chosen for further examination. In situ localization showed it to be expressed in both the myocardium and endothelium. Several antisense DNA probes and an siRNA for latrophilin-2 produced a loss of EMT in collagen gel cultures. Latrophilin-2 is a putative G-protein-coupled receptor and we previously identified a pertussis toxin-sensitive G-protein signal transduction pathway. Microarray experiments were performed to examine whether these molecules were related. After treatment with antisense DNA against latrophilin-2, expression of 1,385 genes and ESTs was altered. This represented approximately 12.5{\%} of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9{\%}) elements of the array. There appears to be little overlap between the two signal transduction pathways. Latrophilin-2 is thus a novel component of EMT and provides a new avenue for investigation of this cellular process.",
keywords = "Cardiac cushions, Cardiac development, EMT, Microarray, Pertussis toxin, TGF-beta, Valvulosepatal development",
author = "Doyle, {Sally E.} and Scholz, {Matthew J.} and Greer, {Kevin A.} and Hubbard, {Antony D.} and Darnell, {Diana K.} and Antin, {Parker B} and Klewer, {Scott E} and Runyan, {Raymond B}",
year = "2006",
month = "12",
doi = "10.1002/dvdy.20973",
language = "English (US)",
volume = "235",
pages = "3213--3221",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "12",

}

TY - JOUR

T1 - Latrophilin-2 is a novel component of the epithelial-mesenchymal transition within the atrioventricular canal of the embryonic chicken heart

AU - Doyle, Sally E.

AU - Scholz, Matthew J.

AU - Greer, Kevin A.

AU - Hubbard, Antony D.

AU - Darnell, Diana K.

AU - Antin, Parker B

AU - Klewer, Scott E

AU - Runyan, Raymond B

PY - 2006/12

Y1 - 2006/12

N2 - Endothelial cells in the atrioventricular canal of the heart undergo an epithelial-mesenchymal transition (EMT) to form heart valves. We surveyed an on-line database (http://www.geisha.arizona.edu/) for clones expressed during gastrulation to identify novel EMT components. One gene, latrophilin-2, was identified as expressed in the heart and appeared to be functional in EMT. This molecule was chosen for further examination. In situ localization showed it to be expressed in both the myocardium and endothelium. Several antisense DNA probes and an siRNA for latrophilin-2 produced a loss of EMT in collagen gel cultures. Latrophilin-2 is a putative G-protein-coupled receptor and we previously identified a pertussis toxin-sensitive G-protein signal transduction pathway. Microarray experiments were performed to examine whether these molecules were related. After treatment with antisense DNA against latrophilin-2, expression of 1,385 genes and ESTs was altered. This represented approximately 12.5% of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9%) elements of the array. There appears to be little overlap between the two signal transduction pathways. Latrophilin-2 is thus a novel component of EMT and provides a new avenue for investigation of this cellular process.

AB - Endothelial cells in the atrioventricular canal of the heart undergo an epithelial-mesenchymal transition (EMT) to form heart valves. We surveyed an on-line database (http://www.geisha.arizona.edu/) for clones expressed during gastrulation to identify novel EMT components. One gene, latrophilin-2, was identified as expressed in the heart and appeared to be functional in EMT. This molecule was chosen for further examination. In situ localization showed it to be expressed in both the myocardium and endothelium. Several antisense DNA probes and an siRNA for latrophilin-2 produced a loss of EMT in collagen gel cultures. Latrophilin-2 is a putative G-protein-coupled receptor and we previously identified a pertussis toxin-sensitive G-protein signal transduction pathway. Microarray experiments were performed to examine whether these molecules were related. After treatment with antisense DNA against latrophilin-2, expression of 1,385 genes and ESTs was altered. This represented approximately 12.5% of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9%) elements of the array. There appears to be little overlap between the two signal transduction pathways. Latrophilin-2 is thus a novel component of EMT and provides a new avenue for investigation of this cellular process.

KW - Cardiac cushions

KW - Cardiac development

KW - EMT

KW - Microarray

KW - Pertussis toxin

KW - TGF-beta

KW - Valvulosepatal development

UR - http://www.scopus.com/inward/record.url?scp=33845356457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845356457&partnerID=8YFLogxK

U2 - 10.1002/dvdy.20973

DO - 10.1002/dvdy.20973

M3 - Article

C2 - 17016846

AN - SCOPUS:33845356457

VL - 235

SP - 3213

EP - 3221

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 12

ER -