Leaf cuticular waxes of lettuce are associated with reduced attachment of the foodborne pathogen Salmonella spp. at harvest and after postharvest storage

Kang Mo Ku, Yu Chun Chiu, Cangliang Shen, Matthew Jenks

Research output: Contribution to journalArticle

Abstract

The amount and composition of cuticular wax on bolting lettuce leaves of different maturity was shown to be associated with Salmonella attachment and survival. The amount of cuticular wax on leaves from the top (40.9 ± 4.7 μg cm−2) and middle stem (44.7 ± 2.9 μg cm−2) locations was significantly greater than that observed on those from the bottom area (17.5 ± 11.9 μg cm−2). Salmonella attachment was compared between leaves from the middle of the lettuce stem (a visible waxy layer) and mature leaves from the lettuce rosette (lacking with a visible waxy layer) to assess the impact of these crystals. Mature rosette leaves on bolting lettuce showed significantly higher Salmonella attachment (5.72 ± 0.16 CFU g−1) than immature leaves (5.18 ± 0.30 CFU g−1) at harvest. After 7 days of storage at 4 °C, the Salmonella survival rate on mature leaves (5.16 ± 0.23 CFU g−1) was significantly higher (P = 0.01) than that observed on immature leaves (4.38 ± 0.33 CFU g−1). Hexacosanol was identified as the major component of platelet-shaped crystals in the recrystallized wax, which were similar to the wax structures on the lettuce leaf surface. These results suggest that the presence of wax crystals may affect Salmonella attachment on lettuce and that their primary component is hexacosanol.

Original languageEnglish (US)
Article number108657
JournalLWT
Volume117
DOIs
StatePublished - Jan 2020

Fingerprint

Lettuce
Salmonella
epicuticular wax
Waxes
Pathogens
food pathogens
lettuce
leaves
waxes
crystals
Crystals
leaf lettuce
bolting
immatures
Platelets
stems
Blood Platelets
survival rate
Chemical analysis

Keywords

  • Epicuticular wax
  • Food pathogen
  • Hexacosanol
  • Lettuce

ASJC Scopus subject areas

  • Food Science

Cite this

Leaf cuticular waxes of lettuce are associated with reduced attachment of the foodborne pathogen Salmonella spp. at harvest and after postharvest storage. / Ku, Kang Mo; Chiu, Yu Chun; Shen, Cangliang; Jenks, Matthew.

In: LWT, Vol. 117, 108657, 01.2020.

Research output: Contribution to journalArticle

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