Leukocyte effects on the dimorphism of Coccidioides immitis

John N Galgiani, R. Hayden, C. M. Payne

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To assess the influences of leukocytes on the dimorphism of Coccidioides immitis, mycelial elements (arthroconidia) or spherules were incubated with polymorphonuclear leukocytes (PMNLs) or mononuclear leukocytes. In the presence of human leukocytes, arthroconidia became septated spherules as determined by electron microscopy but grew as mycelia when viable leukocytes were absent. Plasma was not required for this effect. In a new assay, PMNLs, lymphocytes, or monocytes from human blood as well as rabbit alveolar macrophages inhibited spherules from forming germ tubes. In flatbottomed glassware, maximal inhibition occurred with PMNL:spherule ratios above 15:1, whereas in conical-bottomed glassware a ratio of 3:1 was maximally suppressive with PMNLs as well as mononuclear leukocytes. Cytochalasin B and colchicine blocked the effect of PMNLs but not that of other blood leukocytes on spherules. These studies indicate that leukocytes influence coccidioidal dimorphism toward the spherule phase. This effect may be important in host defenses against C. immitis.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalJournal of Infectious Diseases
Volume146
Issue number1
StatePublished - 1982

Fingerprint

Coccidioides
Neutrophils
Leukocytes
Mononuclear Leukocytes
Cytochalasin B
Mycelium
Alveolar Macrophages
Colchicine
Monocytes
Electron Microscopy
Lymphocytes
Rabbits

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Leukocyte effects on the dimorphism of Coccidioides immitis. / Galgiani, John N; Hayden, R.; Payne, C. M.

In: Journal of Infectious Diseases, Vol. 146, No. 1, 1982, p. 56-63.

Research output: Contribution to journalArticle

Galgiani, John N ; Hayden, R. ; Payne, C. M. / Leukocyte effects on the dimorphism of Coccidioides immitis. In: Journal of Infectious Diseases. 1982 ; Vol. 146, No. 1. pp. 56-63.
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