Leukocyte expression profiles reveal gene sets with prognostic value for seizure-free outcome following stereotactic laser amygdalohippocampotomy

Ryan Sprissler, Robert Bina, Willard Kasoff, Marlys H Witte, Michael J Bernas, Christina Walter, David M Labiner, Branden Lau, Michael F Hammer, Martin E Weinand

Research output: Contribution to journalArticle

Abstract

Among patients with intractable epilepsy, the most commonly performed surgical procedure is craniotomy for amygdalohippocampectomy (AH). Stereotactic laser amygdalohippocampotomy (SLAH) has also been recently employed as a minimally invasive treatment for intractable temporal lobe epilepsy (TLE). Among patients treated with AH and SLAH approximately 65% and 54% of patients become seizure-free, respectively. Therefore, selection criteria for surgical candidates with improved prognostic value for post-operative seizure-free outcome are greatly needed. In this study, we perform RNA sequencing (RNA-Seq) on whole blood leukocyte samples taken from 16 patients with intractable TLE prior to SLAH to test the hypothesis that pre-operative leukocyte RNA expression profiles are prognostic for post-operative seizure outcome. Multidimensional scaling analysis of the RNA expression data indicated separate clustering of patients with seizure free (SF) and non-seizure-free (NSF) outcomes. Differential expression (DE) analysis performed on SF versus NSF groups revealed 24 significantly differentially expressed genes (≥2.0-fold change, p-value < 0.05, FDR <0.05). Network and pathway analyses identified differential activation of pathways involved in lipid metabolism, morphology of oligodendrocytes, inflammatory response, and development of astrocytes. These results suggest that pre-operative leukocyte expression profiles have prognostic value for seizure outcome following SLAH.

Original languageEnglish (US)
Article number1086
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Transcriptome
Seizures
Lasers
Leukocytes
Temporal Lobe Epilepsy
RNA
RNA Sequence Analysis
Craniotomy
Oligodendroglia
Lipid Metabolism
Astrocytes
Patient Selection
Cluster Analysis
Genes
Drug Resistant Epilepsy

ASJC Scopus subject areas

  • General

Cite this

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title = "Leukocyte expression profiles reveal gene sets with prognostic value for seizure-free outcome following stereotactic laser amygdalohippocampotomy",
abstract = "Among patients with intractable epilepsy, the most commonly performed surgical procedure is craniotomy for amygdalohippocampectomy (AH). Stereotactic laser amygdalohippocampotomy (SLAH) has also been recently employed as a minimally invasive treatment for intractable temporal lobe epilepsy (TLE). Among patients treated with AH and SLAH approximately 65{\%} and 54{\%} of patients become seizure-free, respectively. Therefore, selection criteria for surgical candidates with improved prognostic value for post-operative seizure-free outcome are greatly needed. In this study, we perform RNA sequencing (RNA-Seq) on whole blood leukocyte samples taken from 16 patients with intractable TLE prior to SLAH to test the hypothesis that pre-operative leukocyte RNA expression profiles are prognostic for post-operative seizure outcome. Multidimensional scaling analysis of the RNA expression data indicated separate clustering of patients with seizure free (SF) and non-seizure-free (NSF) outcomes. Differential expression (DE) analysis performed on SF versus NSF groups revealed 24 significantly differentially expressed genes (≥2.0-fold change, p-value < 0.05, FDR <0.05). Network and pathway analyses identified differential activation of pathways involved in lipid metabolism, morphology of oligodendrocytes, inflammatory response, and development of astrocytes. These results suggest that pre-operative leukocyte expression profiles have prognostic value for seizure outcome following SLAH.",
author = "Ryan Sprissler and Robert Bina and Willard Kasoff and Witte, {Marlys H} and Bernas, {Michael J} and Christina Walter and Labiner, {David M} and Branden Lau and Hammer, {Michael F} and Weinand, {Martin E}",
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AU - Sprissler, Ryan

AU - Bina, Robert

AU - Kasoff, Willard

AU - Witte, Marlys H

AU - Bernas, Michael J

AU - Walter, Christina

AU - Labiner, David M

AU - Lau, Branden

AU - Hammer, Michael F

AU - Weinand, Martin E

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