Level of expression of IL-13Rα2 impacts receptor distribution and IL-13 signaling

Michael O Daines, Yasuhiro Tabata, Bradley A. Walker, Weiguo Chen, Manoj R. Warrier, Saswata Basu, Gurjit K. Khurana Hershey

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

IL-13, a critical cytokine for allergic inflammation, exerts its effects through a complex receptor system including IL-4Rα, IL-13Rα1, and IL-13Rα2. IL-4Rα and IL-13Rα1 form a heterodimeric signaling receptor for IL-13. In contrast, IL-13Rα2 binds IL-13 with high affinity but does not signal. IL-13Rα2 exists on the cell surface, intracellularly, and in soluble form, but no information is available regarding the relative distributions of DL-13Rα2 among these compartments, whether the compartments communicate, and how the relative expression levels impact IL-13 responses. Herein, we investigated the distribution of IL-13Rα2 in transfected and primary cells, and we evaluated how the total level of IL-13Rα2 expression impacted its distribution. Our results demonstrate that the distribution of IL-13Rα2 is independent of the overall level of expression. The majority of the IL-13Rα2 protein existed in intracellular pools. Surface IL-13Rα2 was continually released into the medium in a soluble form, yet surface expression remained constant supporting receptor trafficking to the cell surface. IL-13Rα2 inhibited IL-13 signaling proportionally to its level of expression, and this inhibition could be overcome with high concentrations of IL-13.

Original languageEnglish (US)
Pages (from-to)7495-7501
Number of pages7
JournalJournal of Immunology
Volume176
Issue number12
StatePublished - Jun 15 2006
Externally publishedYes

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Interleukin-13 Receptors
Interleukin-13
Cytokines
Inflammation
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Daines, M. O., Tabata, Y., Walker, B. A., Chen, W., Warrier, M. R., Basu, S., & Khurana Hershey, G. K. (2006). Level of expression of IL-13Rα2 impacts receptor distribution and IL-13 signaling. Journal of Immunology, 176(12), 7495-7501.

Level of expression of IL-13Rα2 impacts receptor distribution and IL-13 signaling. / Daines, Michael O; Tabata, Yasuhiro; Walker, Bradley A.; Chen, Weiguo; Warrier, Manoj R.; Basu, Saswata; Khurana Hershey, Gurjit K.

In: Journal of Immunology, Vol. 176, No. 12, 15.06.2006, p. 7495-7501.

Research output: Contribution to journalArticle

Daines, MO, Tabata, Y, Walker, BA, Chen, W, Warrier, MR, Basu, S & Khurana Hershey, GK 2006, 'Level of expression of IL-13Rα2 impacts receptor distribution and IL-13 signaling', Journal of Immunology, vol. 176, no. 12, pp. 7495-7501.
Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S et al. Level of expression of IL-13Rα2 impacts receptor distribution and IL-13 signaling. Journal of Immunology. 2006 Jun 15;176(12):7495-7501.
Daines, Michael O ; Tabata, Yasuhiro ; Walker, Bradley A. ; Chen, Weiguo ; Warrier, Manoj R. ; Basu, Saswata ; Khurana Hershey, Gurjit K. / Level of expression of IL-13Rα2 impacts receptor distribution and IL-13 signaling. In: Journal of Immunology. 2006 ; Vol. 176, No. 12. pp. 7495-7501.
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