Levels of detection of tumor cells in human bone marrow with or without prior culture

S. S. Joshi, Deborah J Novak, L. Messbarger, V. Maitreyan, D. D. Weisenburger, J. G. Sharp

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

With an in vitro culture technique combined with light microscopy, immunocytochemistry and molecular probing, we previously detedted occult tumor cells in histologically-normal human bone marrow harvested for autologous transplantation. In this study, we mixed known numbers of malignant lymphoid (Raji and CEM) or breast cancer (MCF-7) cells with normal human bone marrow cells to determine the levels at which tumor cells can be detected before and after culture. Cytocentrifuge preparations were made before culture and after 2 or more weeks of culture and examined by light microscopy. We detected contaminating lymphoma cells at a level of more than 5% before culture, and at a level of 0.01% after culture for 2 or more weeks in 2% human lymphocyte conditioned medium. Before culture, we detected MCF-7 cells at a level of 0.001% using glucose oxidase immunocytochemical staining techniques; these cells were detected at a level of 0.00001% after culture. Since, of necessity, these calibrations were performed using cell lines, it is likely that these results overestimate the absolute sensitivity of these methods for detection of tumor cells in patient samples. We found the glucose oxidase immunocytochemical method more specific for detecting occult tumor cells in bone marrow than the immunoperoxidase staining method because of the absence of non-specific staining arising from endogenous peroxidase in bone marrow cells which makes the interpretation of the latter difficult. We conclude that culture techniques can increase the sensitivity of detection of occult tumor cells in human bone marrow about 100-fold.

Original languageEnglish (US)
Pages (from-to)179-183
Number of pages5
JournalBone Marrow Transplantation
Volume6
Issue number3
StatePublished - Jan 1 1990
Externally publishedYes

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Bone Marrow
Bone Marrow Cells
Culture Techniques
Neoplasms
Glucose Oxidase
MCF-7 Cells
Staining and Labeling
Microscopy
Light
Autologous Transplantation
Conditioned Culture Medium
Calibration
Peroxidase
Lymphoma
Immunohistochemistry
Lymphocytes
Breast Neoplasms
Cell Line

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Joshi, S. S., Novak, D. J., Messbarger, L., Maitreyan, V., Weisenburger, D. D., & Sharp, J. G. (1990). Levels of detection of tumor cells in human bone marrow with or without prior culture. Bone Marrow Transplantation, 6(3), 179-183.

Levels of detection of tumor cells in human bone marrow with or without prior culture. / Joshi, S. S.; Novak, Deborah J; Messbarger, L.; Maitreyan, V.; Weisenburger, D. D.; Sharp, J. G.

In: Bone Marrow Transplantation, Vol. 6, No. 3, 01.01.1990, p. 179-183.

Research output: Contribution to journalArticle

Joshi, SS, Novak, DJ, Messbarger, L, Maitreyan, V, Weisenburger, DD & Sharp, JG 1990, 'Levels of detection of tumor cells in human bone marrow with or without prior culture', Bone Marrow Transplantation, vol. 6, no. 3, pp. 179-183.
Joshi SS, Novak DJ, Messbarger L, Maitreyan V, Weisenburger DD, Sharp JG. Levels of detection of tumor cells in human bone marrow with or without prior culture. Bone Marrow Transplantation. 1990 Jan 1;6(3):179-183.
Joshi, S. S. ; Novak, Deborah J ; Messbarger, L. ; Maitreyan, V. ; Weisenburger, D. D. ; Sharp, J. G. / Levels of detection of tumor cells in human bone marrow with or without prior culture. In: Bone Marrow Transplantation. 1990 ; Vol. 6, No. 3. pp. 179-183.
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