Light-Activated Triazabutadienes for the Modification of a Viral Surface

Stephanie M. Jensen, Flora W. Kimani, John C. Jewett

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host–pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.

Original languageEnglish (US)
Pages (from-to)2216-2219
Number of pages4
JournalChemBioChem
Volume17
Issue number23
DOIs
StatePublished - Dec 2 2016

Keywords

  • aryl diazonium ion
  • bioconjugation
  • crosslinking
  • photochemistry
  • viruses

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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