Light-triggered and cysteine-mediated nitric oxide release from a biodegradable starch-based film

Antonio Carlos Roveda, Helena De Fazio Aguiar, Katrina M Miranda, Carmen Cecília Tadini, Douglas Wagner Franco

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A new nitric oxide-releasing material produced with cassava starch is described. The ruthenium nitrosyl complex trans-[Ru(NH3)4(isn)NO](BF4)3 (RuNOisn; isn = isonicotinamide) is able to release NO upon either photolysis or chemical reduction. Impregnating this complex under mild conditions into cassava starch (CS) films produced a NO-delivery platform (CSx-RuNOisn). Spectroscopic analysis of CSx-RuNOisn indicates that the coordination sphere of RuNOisn remains intact during film production. Exposure of CSx-RuNOisn to long wave UV-light (λirr = 355 nm) leads to NO release and formation of the paramagnetic photoproduct trans-[RuIII(NH3)4isn(H2O)]3+ in the CS film. Reaction of this aquaruthenium(iii) complex with aqueous nitrite regenerates RuNOisn in the film. Delivery of NO upon photolysis of CSx-RuNOisn was verified by trapping with oxymyoglobin. Moreover, NO release upon chemical reduction was carried out using l-cysteine as a reductant. Cysteine-mediated NO delivery from CSx-RuNOisn persisted for more than 7 h, during which physiologically relevant NO concentrations were liberated. These results suggest that CSx-RuNOisn is a promising candidate for use in biological applications.

Original languageEnglish (US)
Pages (from-to)7232-7242
Number of pages11
JournalJournal of Materials Chemistry B
Volume2
Issue number41
DOIs
StatePublished - Nov 7 2014

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Manihot
Nitric oxide
Starch
Cysteine
Nitric Oxide
Photolysis
Light
Ruthenium
Reducing Agents
Ultraviolet Rays
Nitrites
Spectroscopic analysis
Ultraviolet radiation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)
  • Chemistry(all)
  • Materials Science(all)

Cite this

Light-triggered and cysteine-mediated nitric oxide release from a biodegradable starch-based film. / Roveda, Antonio Carlos; De Fazio Aguiar, Helena; Miranda, Katrina M; Tadini, Carmen Cecília; Franco, Douglas Wagner.

In: Journal of Materials Chemistry B, Vol. 2, No. 41, 07.11.2014, p. 7232-7242.

Research output: Contribution to journalArticle

Roveda, Antonio Carlos ; De Fazio Aguiar, Helena ; Miranda, Katrina M ; Tadini, Carmen Cecília ; Franco, Douglas Wagner. / Light-triggered and cysteine-mediated nitric oxide release from a biodegradable starch-based film. In: Journal of Materials Chemistry B. 2014 ; Vol. 2, No. 41. pp. 7232-7242.
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