Limits of detection of chemopreventive efficacy: Karyometry of skin biopsies

Peter H. Bartels, Michael L. Yozwiak, Hubert G. Bartels, Yun Liu, Lisa M. Hess, David S. Alberts

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Objective: This study was designed to establish estimates of the smallest effects due to chemopreventive intervention detectable by karyometry in skin biopsies. Methods: Estimates of the smallest change of statistical significance and estimates of the power of the test were derived for several key features descriptive of the distribution of nuclear chromatin. Results from triplicate biopsies from the same case were used to provide estimates of the within-case, biopsy-to-biopsy variance. Results: Generally, a change in feature value due to chemopreventive intervention can be statistically secured when it amounts to 5% to 10%. In clinical trials where matched baseline and end of study biopsies from the same cases are available, paired comparison ANOVA can detect a 2% change on samples of 25 cases. Establishing efficacy in individual cases requires a change in feature values on the order of 10% to 15%. Conclusions: Karyometry provides a sensitive, quantitative method for the assessment of efficacy of chemoprevention. The effects of within-case, biopsy-to-biopsy variance need to be considered only in the evaluation of individual cases and are on the order of 5% in skin biopsies.

Original languageEnglish (US)
Pages (from-to)1689-1695
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Issue number7
StatePublished - Jul 2008

ASJC Scopus subject areas

  • Epidemiology
  • Oncology


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